Title : Cheap heterogeneous phyto-antibiotics may solve the drug-void against superbugs: Purification of phyto-chemical and identification of targets as RNA polymerase and DNA topoisomerase
Phage therapy and nanodrug carriers are most vital area of modern research against multidrug-resistant bacteria. Further, enzybiotics and gene medicines (antisense, ribozyme and siRNA) research have focused great but no clinical trials approved yet. Soil bacteria have maintained plant world who secret anti-metabolites against soil bacteria, being used >5000 years back to cure many diseases as described Indian Sanskrit books like Charaka Samhita, Sasruta Samhita and Atharva Veda. Today plant’s active principles have purified and structural analysis were performed and thus quinine, artiminisin, taxol, reserpine, camptothecin were well respected antimalarial, anti-cancer and antibacterial drugs. As antibiotic void is now clear with millions of mdr genes have been sequenced with subsequent purification of enzymes and determination of crystal structures. We have described here our efforts to make plant derived active chemicals as drugs against superbugs. Presently multi-resistance in bacteria has focused so importantly that WHO and G-20 nations have agreed to discover alternate to conventional new antibiotics that are quickly getting resistant after use of few months. Our estimates are 40% of all river and sea water bacteria near the big cities are ampicillin and to lesser extent tetracycllne, azithromycin, ciprofloxacin and streptomycin resistant. Whereas <1% are superbugs and ~0.002% is meropenem resistant. Amp, neo, tet, aac, cat, aph, aad, mcr-1, blaNDM-1, blaKPC-1, arr3, sul1/2/3, dhfr, inh, acrAB, mexAB/CD/EF, macAB, mtrCDE are abundant with millions mutated isomers. Drug resistance is ubiquitous and new gene creation is everywhere as 50-500kb MDR plasmids carry >15 unknown genes and numerous transposons to fight against complex antibiotics. Gut bacteria like Escherichia coli has acquired many mdr genes in plasmids as also in Klebsiella pneuoniae, Salmonella enterica and Pseudomonas aeruginosa and Acinetobacter baumannii. Chromosomal drug efflux genes (acrAB, mexAB/CD/EF, macAB, mtrCD and norA) are found in many pathogens including Mycobacterium tuberculosis and Neisseria gonorrhoeae. Problem has occurred as we have not taken enough probiotics and vitamins during antibiotics uses and due to multi-resistance, we are constantly killing gut bacteria which synthesis vitamins required for >30,000 enzymatic reactions in human metabolosome. Isolated superbugs were resistant to ampicillin, tetracycline, streptomycin, chloramphenicol, ciprofloxacin, azithromycin, vancomycin, amikacin, meropenem but sensitive to organic extract of Suregada multiflora roots, Cassia fistula bark, Jatropha gossypiifolia roots as well as extracts from Indian spices Labanga and Derchini (MDR-Cure). TLC and HPLC purification as well MASS, NMR, FT-IR and XRD-Powder gave many distinct signatures of pure chemicals to inhibit multidrug-resistant bacteria. We found that NU2 inhibits E coli DNA topiisomeraseI and CU1 inhibits E . coli and Mycobacterium tuberculosis RNA polymerases. Back to Nature: Save Plants and Use as Medicine.
Audience Take Away:
- Explain how the audience will be able to use what they learn?
- How will this help the audience in their job? Is this research that other faculty could use to expand their research or teaching? Does this provide a practical solution to a problem that could simplify or make a designer’s job more efficient? Will it improve the accuracy of a design, or provide new information to assist in a design problem? List all other benefits.
- Audience will learn how to collect .plant parts and how to extract it and how to assay on MDR bacteria.
- How to purify phyto-chemical with no great facility in poor countries and then how to collaborate with good scientists for better knowledge.
- The lecture will revive the lost trust of phyto-medicne and the important roles of roles of herbalers in ancient Indian and Chinese civilization.
- Leaders will then know how to save medicinal plants and use them as drugs.