Title : The role of highly active antiretroviral therapy (haart) on interleukin 17a (il-17a) in normotensive and pre-eclamptic black South African women
Introduction: Interleukin 17-A (IL-17A) has been implicated to be involved in the pathophysiology of both human immune deficiency virus (HIV) and preeclampsia (PE). This study evaluated the serum levels of IL-17A in HIV-negative and HIV-positive normotensive and pre-eclamptic women receiving highly active anti -retroviral therapy (HAART).
Material and methods: A total sample size of n=250 was used. Normotensive women (n=150; N), preeclamptic women (n=100; PE). Normotensive women were further stratified into HIV negative (n=90), HIV positive (HAART acute) (n=30) and HIV positive (HAART chronic) (n=30) women. Pre-eclamptic women were divided into early onset (n=50; EOPE) and late-onset (n=50; LOPE). The EOPE and LOPE groups were subdivided into HIV negative (n=30), HIV positive (HAART acute) (n=10), and HIV positive (HAART) chronic (n=10). Analysis of IL-17A was performed using the multiple Bio-Plex immunoassay method.
Results: With regards pregnancy type, based on HIV negative status;
The levels of IL-17A were significantly increased in N compared to LOPE (P= 0.0429). No significant difference was noted between N vs EOPE and EOPE vs LOPE.
With regards pregnancy type, based on HAART duration (HIV positive HAART chronic);
The levels of IL-17A were significantly increased in the N compared to the EOPE (P= 0.0129) groups. No significant difference was noted between N vs LOPE and EOPE vs LOPE.
With regards HIV status, based on normotensive groups;
The levels of IL-17A were significantly increased in HIV positive (HAART-chronic) compared to HIV negative (P=0.0299) and in HIV positive (HAART-chronic) compared to HIV positive (HAART-acute) (P=0.0150).
With regards EOPE and the LOPE groups, based on HIV status;
There was no significant difference in the levels of IL-17A amongst all groups.
Conclusion: The study demonstrates that IL-17A is involved in maintaining normal pregnancy and that reduction in IL-17A is involved in the pathophysiology of PE. Our findings also indicate that upregulation of IL-17A as a result of prolonged exposure to HAART is not associated with PE development, however exaggerative activation of other cytokines might be the cause.
Audience Take Away:
- Improve Understating on the pathophysiology of pre-eclampsia in HIV associated pregnancies by with regards the levels of IL-17A in normotensives compared to early onset and late onset re-eclamptic
- The level of IL-17A can improve their knowledge on the predictive risk indicators of pre-eclampsia in HIV-associated pregnancies
- Other faculty can expand to this research as by looking at the other ethnic groups and also by looking at the placental tissue instead of serum used in the current study in order to validate the current findings.