Abstract:
This presentation will discuss the aim of the study; to compare the staining specificity and intensity of Hematoxylin and Eosin (H&E) stain and Modified Papanicolaou stain, to stain the keratin present in Oral Squamous Cell Carcinoma. It will also discuss the methodology used to implement the study; an analytical cross-sectional study was conducted with different samples (n = 110) of oral squamous cell carcinoma. Duplicate sections were obtained from each block, and one section was stained with H&E and the other with Modified Papanicolaou. The stained sections were then evaluated based on the specificity and intensity of staining keratin using modified scoring criteria of poor satisfactory good and excellent. Then it will discuss the results obtained by the study; the staining specificity and intensity of Modified Papanicolaou stain were significantly higher than those of H&E (P = 0.0000001 and P = 0.00002, respectively). Finally, it will discuss how this new modified technique will improve the diagnosis; Modified Papanicolaou stain exhibited easier diagnosis of keratinization in samples of oral squamous cell carcinoma than H&E stain, because it provided better contrast to the background, allowing easy identification of keratin and more accurate diagnosis. Moreover, this modified method can be adapted for precise diagnosis of other tissues that form keratin in the presence of cancer, which can in turn help determine the appropriate treatment.
