ICC 2019

Nusyba Mohamed Ahmed Bushra Mohamed Ahmed

Nusyba Mohamed Ahmed Bushra Mohamed Ahmed, Speaker at  Oncology Conferences
University of Medical Sciences and Technology, Sudan
Title : Comparison between modified papanicolaou stain and hematoxylin eosin stain for staining keratin in oral tissue sections

Abstract:

This presentation will discuss the aim of the study; to compare the staining specificity and intensity of Hematoxylin and Eosin (H&E) stain and Modified Papanicolaou stain, to stain the keratin present in Oral Squamous Cell Carcinoma. It will also discuss the methodology used to implement the study; an analytical cross-sectional study was conducted with different samples (n = 110) of oral squamous cell carcinoma. Duplicate sections were obtained from each block, and one section was stained with H&E and the other with Modified Papanicolaou. The stained sections were then evaluated based on the specificity and intensity of staining keratin using modified scoring criteria of poor satisfactory good and excellent. Then it will discuss the results obtained by the study; the staining specificity and intensity of Modified Papanicolaou stain were significantly higher than those of H&E (P = 0.0000001 and P = 0.00002, respectively). Finally, it will discuss how this new modified technique will improve the diagnosis; Modified Papanicolaou stain exhibited easier diagnosis of keratinization in samples of oral squamous cell carcinoma than H&E stain, because it provided better contrast to the background, allowing easy identification of keratin and more accurate diagnosis. Moreover, this modified method can be adapted for precise diagnosis of other tissues that form keratin in the presence of cancer, which can in turn help determine the appropriate treatment.

Biography:

MS. Nusyba Bushra is an outstanding Biomedical Scientist. She received her B.Sc. from University of Medical Sciences & Technology (UMST), with an overall GpA of 4.97 out of 5.00. She had been awarded many prizes and scholarships during her academic years at the university including; University’s scholarship for the year 2014 – 2015 and the year 2015 – 2016, University’s prize in Histopathology and University’s prize for the Best overall performance. Then she followed it by a higher educational diploma in Research Methodology & Biostatistics, and implemented a research with her diploma’s supervisor doctor Mounkaila Noma

Watsapp