Scientific Program

Abstract:

Breast cancer (BC) is the most commonly diagnosed cancer in women in the world. Many studies have shown that phenolic acids are anticancer agents. 2,3-dihydroxybenzoic acid (2,3-DHBA) is a natural phenolic acid found in different fruits or vegetables. Lipidomic approaches investigate changes in lipid types in both normal and pathological conditions. Various diseases, including cancer, have been shown to be associated with specific changes in certain types of lipids, and new treatment strategies involving lipid metabolism have been developed. However, no studies have been conducted to show the effects of 2,3-DHBA on the lipid species of MCF-7 human breast cancer cells. The in vitro anticancer activity of 2,3-DHBA (0.75-20 mM) against MCF-7 cancer cells and L-929 healthy mouse fibroblast cells determined by MTT (3- (4,5-dimethylthiazol-2-yl) -2,5-diphenyltetrazolium) method for 24h, 48h and 72h. 2,3 DHBA was found to have anticancer activity on MCF-7 cancer cells and this effect increased in a time and dose dependent manner. Our results indicates that the 2,3-DHBA more cytotoxic against breast cancer cells than L-929 healthy cells. The effects of 2,3 DHBA on the lipid profile of MCF-7 cancer cells were studied using the untargeted lipidomic approach for the first time. It was determined that incubation of MCF-7 cancer cells with 2,3 DHBA caused changes in glycerophospholipid, sphingolipid, glycerolipid and fatty acid lipid species. As a result, the relationship between the in vitro anticancer activity of 2,3-DHBA and the differentiation of the lipid profiles of MCF-7 breast cancer cells through this activity was determined.
“This work is supported by the Scientific Research Project Fund of Sivas Cumhuriyet University under the project number ECZ-049”.

Audience Take Away Notes:

• The audience will gain insight into the role of 2,3-DHBA on lipid profile changes of MCF-7 breast cancer cells
• The audience will gain insight into lipidomic techniques in cancer studies
• Other faculty may shape their future research on effects of natural compounds on lipid metabolism in cancer
• The audience may apply the technique mentioned in this speech in their research

Biography:

Assoc. Prof. Dr. Serap Sahin-Bolukbasi completed her Ph.D. studies on Biochemistry at The Sivas Cumhuriyet University. She performed post-doctoral studies at The University of Georgia (UGA) Collage of Pharmacy, Department of Pharmaceutical and Biomedical Sciences. She is an Assoc. Prof. Dr. at the Afyonkarahisar Health Sciences University, Faculty of Pharmacy, Department of Biochemistry since 2021. She has published many research articles in SCI (E) journals and presented more than 80 presentations at national/international conferences.  Dr. Sahin-Bolukbasi’s research focuses on lipidomic, lipid metabolism in cancer, lipid biomarkers for rare and common diseases, new treatment targets based on lipid metabolism, cell culture, development of biotechnological aptamers in cardiovascular diseases and cancer.