Title : Physicochemical, phytochemical, hptlc and antioxidant study of medicinal plant drug Butea monosperma
Abstract:
Butea monosperma (Family Fabaceae) popularly known as ‘dhak’ or ‘palas’, commonly known as ‘Flame of forest’ is an important plant and is extensively utilized for lac cultivation. Butea monosperma is found in countries like India, Bangladesh, Nepal, Pakistan, Sri Lanka, Myanmar, Thailand, Laos, Cambodia, Vietnam, Malaysia, and western Indonesia. The main active constituents of Butea monosperma is oreopsin, isocoreopsin, sulphurein, butein, butin, isobutrin, monospermoside and isomonospermoside, aurones, chalcones, flavonoids (palasitrin, prunetin) and steroids. It has strong antioxidant, hepatoprotective, anthelmintic, anti-diabetic, anti-stress, antifungal, astringent, aphrodisiac, laxative properties. It acts as anti-diarrheal and anti-inflammatory drug. Butea monosperma traditionally used for the treatment of piles, eye diseases, inflammation, diseases of the anus, dysentery, hydrocele, ulcers, tumours, dysmenorrhea, liver disorder, gonorrhoea and it also purifies the blood. Butea monosperma flower and seeds are consumed by children as remedy against intestinal worms. Its leaf & flower used as an appetizer, astringent, carminative, anthelmintic, aphrodisiac, tonic, lessens inflammation and lumbago, cures boils and piles. Also helps to cure cough, cold and stomach disorders. Butea monosperma gum is astringent to bowel, good in stomatitis, cough, pterygium, corneal opacities and cures excessive perspiration, goitre of human being and body swellings. Its bark is acrid, bitter, appetiser, aphrodisiac, laxative, anthelminthic properties. Present study reveals the results of Physiochemical, Phytochemical and High Performance Thin Layer Chromatography study of Butea monosperma flower. The result of Physiochemical study, LOD (2.85%), Total Ash (5.43%), Alcohol extractive values (13.26%), Water extractive values (29.00%), Total solids (3.30%), Wt/ml (0.92g) and Alcohol content (53.2%). In UV Spectroscopy λmax. observed at 325nm in in-house sample. HPTLC analysis of chloroform extract of in-house sample was performed by using toluene ethyl acetate (9:1, v/v) as mobile phase. Visualizing under UV light (254 nm), five spots appear at Rf. 0.21, 0.32, 0.65 and 0.71 (all brown). Under U.V light (366nm), six spots appear at Rf. 0.21(brown), 0.28(yellow), 0.32(brown), 0.48(blue), 0.60(blue) and 0.70(brown). After derivatization with Anisaldehyde Sulphuric acid reagent at 366 nm five spots appear at Rf. 0.21, 0.32 , 0.42, 0.68 and 0.70 (all blue) which confirms the presence of active constituent in Butea monosperma flower extract. The present physicochemical, phytochemical and HPTLC data are to be considered as monograph of pharmacopoeial standards for aforesaid drug.
Audience Take Away Notes:
- Audience will learn about Drug standardization parameter for natural medicinal plant drug for checking safety, therapeutic efficacy and quality of the drug
- The present research will help the industry and researchers and scientist to set parameters for drug standardization study for natural medicinal plant drugs, which will be beneficial to maintain batch to batch accuracy of manufactured drug.
- Yes the present research may open the area of vast research opportunities on medicinal
- Plant biochemistry and become useful for the faculty to expand their research in the field of natural’s medicinal plant chemistry and motivates audience to do further research in natural medicinal plant.
- Yes it helps in the quantitative estimation of natural medicinal plant drugs should be carefully evaluated and quantified to determine the presence of active compounds in these drugs which are responsible for the overall pharmacological activity of the medicine.
- The presence of active constituents in mother tincture suggests that the mother tincture of medicinal plant drugs contained specific active compounds which justify its medicinal
- usage in complementary medicine and the reason for a cure and healing properties of these medicines.
- The present studied plant Butea monosperma is an important plant and is extensively utilized for lac cultivation. Butea monosperma acts as anti-diarrheal and anti-inflammatory drug and traditionally used for the treatment of piles, eye diseases, inflammation, diseases of the anus, dysentery, hydrocele, ulcers, tumours, dysmenorrhea, liver disorder, gonorrhoea and it also purifies the blood.
