Khatereh zarkesh

Title : Theranostic hyaluronan coated edta modified magnetic mesoporous silica nanoparticles for targeted delivery of cisplatin

Abstract:

One of the eminent capabilities of nanoparticles in drug delivery is active targeting. Mesoporous silica nanoparticles (MSNs) are among the well-known nanocarriers, which have been utilized for gene and drug deliveries due to their particular physical and chemical properties.  The present study aimed to introduce an engineered type of EDTA-modified Magnetic Mesoporous Silica Nanoparticles (MMSNs) with hyaluronan coating as a theranostic agent for the delivery of an anti-cancer drug (cisplatin). EDTA was first applied as a ligand for platinum-based drug loading. The nanoparticles were synthesized in several steps. The properties of each step were characterized by various techniques such as Fourier transform infrared (FT-IR), X-Ray diffraction (XRD), vibrating sample magnetometer (VSM), and N₂ adsorption/desorption. Morphology was also checked by field emission scanning electron microscopy (FE-SEM) and Transmission electron microscopy (TEM). In addition, loading capacity (LC) and loading efficiency, and in vivo release of cisplatin were analyzed through ICP-OES. Eventually, the plasma concentration profile and cisplatin pharmacokinetics parameters after intravenous administration of EDTA-MMSN @ HA were evaluated. The average particle diameters of the prepared structure were an approximate range of 70-100 nm. The profile release of cisplatin in an acidic medium was faster in 72 hours, which confirmed the pH-responsive behavior of the nanoparticles. As the results of cytotoxicity showed, coating with HA can improve the internalization of nanoparticles in cancer cells compared to normal cells. Finally, EDTA-MMSN @ HA can be introduced as a new favor biocompatible and biodegradable nanoplatforms for targeted drug delivery which can improve pharmacokinetic parameters. Overall, this study successfully synthesized a novel multifunctional pH-responsive MSN for efficient drug delivery and magnetic resonance imaging.

Audience Take Away Notes:

• Application of nanotechnology in the biopharmaceutical field
• Summarize types of nanocarrier for smart drug delivery systems (DDSs)
• Introduce mesoporous silica nanoparticles (MSN)as a novel structure in biomedical and bioimaging (THERANOSTIC agent)
• Application of magnetic nanoparticles as contrast agents in Magnetic resonance imaging (MRI)
• Advantages of MSN in comparison of other carriers include biocompatibility, high surface area, tunable morphology and pore size, porosity, and adjustable surface functional groups (active targeting).

Biography:

Khatereh zarkesh studied pharmacy at the Kerman university of medical sciences Under and graduated as Pharm.D in 2016. The thesis title has been Preparation and Physicochemical Characterization of Topical Niosomal Formulation of Minoxidil and Tretinoin Under the supervision of Prof. pardakhty and prof. khazaeli. She then joined the research group of Prof. Mohammadi-Samani at the Faculty of Pharmacy, Shiraz University of Medical Sciences, Shiraz, Iran. She received her Ph.D. degree in Pharmaceutics in 2022.  After that, she obtained the position of an Associate Professor at the faculty of pharmacy, at Hormozgan University of Medical Sciences. She has participated in various pharmacy and pharmaceutical science as a Poster presentation. She trained in various workshops including Column Chromatography, Vaccine Design and Production, PCMS academy training molecule and polymerase chain reaction.