Title : Insulinotropic activity of standardized methanolic extracts of seven medicinal plants in Iraq
Abstract:
Seven Iraqi Plants (SIP) a traditional medicinal plant that has been proven to show antidiabetic effects. This study focus is to assess the insulin secretion activity of SIP standardized methanolic extracts and mechanisms that underlie the insulin secretion action of the extracts. The cytotoxicity of SIP extracts was tested using viability assay. The insulin secretion assay was carried out by treating clonal BRIN BD11 cell line with standardized methanolic SIP extracts or glybenclamide. The clonal BRIN BD11 cell was also treated with insulin agonist and antagonist to elucidate the insulin secretion mechanism. Only the viability percentage for SIP galium verum and Madagascar Periwinkle was identified to be toxic at 500 and 1000 g/ml (P<0.001). The insulin secretion for SIP Datura, Basil, and German chamomile was dose-dependent; further evaluation suggested that SIP rheum ribes was involved in KATP-independent pathway. This study suggests that standardized methanolic extracts of SIP have an insulinotropic effect on clonal BRIN BD11 cell line and can be utilized as a modern candidate of antidiabetic agents targeting the escalation for insulin secretion from pancreatic beta cells.
