The rapid evolution of artificial intelligence (AI) is reshaping the landscape of biomedical research and opening new possibilities in the field of regenerative medicine. Regenerative medicine aims to restore, replace, or regenerate damaged tissues and organs through the combined [....] » Read More
Predicting human drug responses from animal studies remains a persistent challenge in pharmaceutical development. Despite extensive optimization of preclinical protocols, fewer than 10% of drug candidates entering clinical trials ultimately receive approval, with failures frequen [....] » Read More
Inherited retinal dystrophies (IRD) impact millions of people and are leading causes of blindness globally. IRDs can be caused by over 300 different genetic mutations and many of the individual disorders are rare diseases with low prevalence and few treatment options. One treatme [....] » Read More
Melanoma and squamous cell carcinoma are among the most common skin cancers worldwide, with rising incidence and significant clinical impact. To enhance the translational relevance of preclinical drug discovery, we developed high-throughput, three-dimensional (3D) bioprinted huma [....] » Read More
Despite substantial advances in scaffold chemistry, architecture, and biofunctionalization, tissue engineering systems continue to be fundamentally constrained by open-loop design paradigms in which cellular responses are assumed to be predictable and static. Such assumptions are [....] » Read More
Articular cartilage has a limited intrinsic capacity for repair, and the development of durable hyaline-like cartilage remains a major challenge in regenerative medicine. Although cell-based therapies using mesenchymal stromal cells, chondrocytes, and cartilage-derived progenitor [....] » Read More
Regulation of cell death plays an important role for cellular adaptation to stress and associated signaling processes. Previous studies suggested that arginylation, a protein post-translational modification, catalyzed by evolutionarily conserved enzyme arginyltransferase 1 (Ate1) [....] » Read More
This study investigated the effects of physical factors induced by magnetite nanoparticles (specifically, constant magnetic fields and sorption activity) on microorganisms belonging to various taxonomic groups. Biomarkers included the intensity of Free Radical Lipid Peroxidation [....] » Read More
Gingival fibroblasts (GFs) represent an abundant cell population of oral connective tissue. Their primary function is production and remodeling of extracellular matrix (ECM) and release of growth factors to maintain tissue integrity and structure, tissue regeneration and wound he [....] » Read More
The convergence of textile science, biomaterials engineering and regenerative medicine is creating new opportunities for the development of functional scaffolds capable of supporting tissue repair and regeneration. Textile materials are particularly attractive for tissue engineer [....] » Read More
Introduction: The functional integrity of limbs depends on healthy bones, tendons, and soft-tissue cover. High-velocity trauma has led to an increase in complex wounds exposing these vital structures, often with extensive devitalization of overlying tissues. This precipitates a c [....] » Read More
Atopic dermatitis (AD) is a chronic inflammatory skin disease characterized by immune dysregulation, epidermal barrier impairment, and defective tissue repair. Because mechanisms involved in barrier restoration overlap with those governing wound healing, biomaterials that simulta [....] » Read More
Background: Mesenchymal Stromal Cells (MSCs) are representing a heterogenous non hematopoietic stem cell population of mesodermal origin, with significant differentiation and immunoregulatory properties. The umbilical cord (UC) represents a perinatal tissue for the isolation of f [....] » Read More
Skeletal muscle has remarkable regeneration capabilities, mainly due to its resident muscle stem cells (Satellite Cells: SCs). Following satellite cell activation, several factors drive asymmetric cell division to generate a stem cell and a proliferative progenitor that forms new [....] » Read More
Investigation of agents that may enhance tissue regeneration in elastic cartilage damage is important for the development of regenerative therapies. Botulinum toxin type A (BTXA) is known for its ability to induce muscle paralysis, but also because it can modulate the inflammator [....] » Read More
Introduction: Peripheral nerve regeneration remains challenged by the limited effectiveness of current clinical repair strategies, including autologous nerve grafting. The development of physiologically relevant in vitro models that reproduce neuron–Schwann cell interaction [....] » Read More
Background: Effective wound healing depends on maintaining a balanced local environment at the wound site. Excessive wound exudate can prolong inflammation, increase infection risk, cause maceration of surrounding tissue, and interfere with cell migration and extracellular matrix [....] » Read More
Title : Advanced 3D tissue models: Pioneering tools for investigating health and disease
Lucie Bacakova, Institute of Physiology of the Czech Academy of Sciences, Czech Republic
In vitro tissue models are laboratory-grown biological systems that use isolated cells, tissues, or organs to simulate the structure and function of living organisms. Traditional models are two-dimensional (2D) cell cultures, in which cells are grown as a monolayer on flat s [....] » Read More
Title : Polysaccharide Based Nanotubes for Medical Applications
Ivan Kelnar, Institute of Macromolecular Chemistry, Czech Academy of Sciences, Czech Republic
β-1,3-glucan polysaccharides are important biomaterials with a comb-like structure, which treat diseases and promote health in a number of important ways including antitumor, immune-stimulating activities, etc. Their well-known triple-helix conformation in water has so far b [....] » Read More
Title : Stem cells for human disease modelling and potential for tissue repair
Patrizia Ferretti, UCL Great Ormond Street Institute of Child Health, United Kingdom
Understanding the mechanisms underlying human disease and translating this knowledge into therapeutic strategies remain major challenges, particularly for congenital disorders that affect multiple tissues and organs. A key issue is the importance of selecting the most appropriate [....] » Read More
Title : 3D in vitro model of Dupuytren’s disease
Jarmila Knitlova, Institute of Physiology of the Czech Academy of Sciences, Czech Republic
Dupuytren’s disease (DD) is a common fibrotic disorder of the hand, characterized by progressive thickening and contracture of the palmar and digital fascia. Surgical excision remains the primary treatment; however, there are currently no therapies to prevent disease progre [....] » Read More
Title : An adipose-derived dECM hydrogel as a versatile ECM platform for pancreatic diseases
Lisa Martin, University Grenoble Alpes, France
Pancreatic ductal adenocarcinoma (PDAC) accounts for approximately 90% of pancreatic cancers and remains one of the deadliest malignancies in the worldwide, with a 5-year survival rate of only 8–13%1–3. PDAC arises from the exocrine compartment (ductal and acinar ce [....] » Read More
Title : Enhanced osteogenic differentiation of human mesenchymal stem cells: Impact of surface terminations of nanocrystalline diamond coatings
Shanaya Surendra Phal Desai, Institute of Physiology of the Czech Academy of Sciences, Czech Republic
The existing bone implant materials lack sufficient mechanical stability and surface properties for effective cell-material interactions, thereby limiting osseointegration. Nanocrystalline diamond (NCD) coatings are promising materials for bone biomimetics because of their compel [....] » Read More
Title : Planar microorganoPit-based co-culture platform (MICA) for studying tumor-immune interactions in patient-derived tumoroids
Xiaochun Cao Ehlker, HTCR-Services GmbH, Martinsried/Planegg, Germany
Mechanistic studies of tumor-immune interactions and preclinical evaluation of immunotherapies require in vitro models that closely recapitulate the tumor microenvironment. Although patient-derived tumor organoids preserve key structural and functional features of native tumors, [....] » Read More
Title : A resorbable and biomimetic 3D-printed natural hydrogel scaffold driving large-volume soft tissue repair: evidence from a large animal study
Audrey Cherblanc, Healshape SAS, France
Breast cancer is the most common form of cancer in women, often requiring total mastectomy. In the US, 38% of affected women undergo this procedure (1), yet only 33% opt for reconstruction (2). Despite the availability of multiple reconstruction methods, a study conducted in the [....] » Read More
Title : MXene-based biomaterials in tissue engineering and functional implant coatings: Recent advances and future prospects
Lucie Bacakova, Institute of Physiology of the Czech Academy of Sciences, Czech Republic
MXenes — two-dimensional transition-metal carbides and nitrides — have emerged as transformative materials in regenerative medicine. These materials, particularly Ti3C2Tx, offer a unique combination of metallic conductivity, hydrophilic surface chemistry, and robust m [....] » Read More
Title : Physics-guided modelling for optimising cell alignment in lab-grown meat scaffolds
Fairooza Alam, The Open University, United Kingdom
The development of lab-grown meat and engineered tissues for healthcare applications is constrained by the challenge of controlling cell organisation within three-dimensional structures. In particular, achieving aligned and functional tissue architecture remains difficult due to [....] » Read More
Title : In vitro evaluation of lyophilized dedifferentiated fat cells (DFAT) impregnated artificial dermis
Kazutaka Soejima, Nihon University, School of Medicine, Japan
Dedifferentiated fat cells (DFAT) were infiltrated into an artificial dermal collagen sponge under reduced pressure, followed by lyophilization to create lyophilized DFAT-impregnated artificial dermis. In this study, the release of bioactive substances was examined in vitro. M [....] » Read More