Title : Investigation impact of PVA nanofibers, collagen-coated, containing the conditioned medium of adipose mesenchymal stem cells (hA-MSCs) in third-degree burn wound healing
Abstract:
Conditioned medium (CM) extracted from human adipose mesenchymal stem cells (hA-MSCs) contains abundant amounts of various growth factors and cytokines, boosts cell viability, proliferation, migration, immunomodulation, and promotes wound healing. However, for practical applications, a biodegradable supporting material is required to maintain CM in the wound area. polyvinyl alcohol (PVA) nanofibers coated with collagen (PVA-Col/CM) are suitable carriers due to their biocompatibility and biodegradability. In this study, CM was derived from cultured hA-MSCs and identified twelve growth factors and cytokines with dominant expression (TNF-α, VEGF, EGF, FGF-2, IGF-1, HGF, PGE2, IL-6, IL-10, IL-1ra, IDO, and HLA-G) that are known to contribute in wound healing. The PVA nanofiber scaffolds were developed to evaluate the relevant factors and conditions in terms of mechanical properties to enhance burn wound regeneration. For this purpose, different concentrations (0.5–1 % w/v) of Col were initially coated onto PVA nanofibers to optimize the suitable carrier due to swelling, biodegradation behavior, and mechanical properties. Adding 1 % w/v Col resulted in optimal mechanical properties and biodegradability. Next, different concentrations (10, 20, and 30 mg/ml) of CM were added to Col 1% to prepare the PVA-Col/CM scaffold. The PVA-Col/CM (30 mg/ml) was selected as the optimum scaffold for burn wound healing, considering cell viability and cytocompatibility. Moreover, implantation of PVA-Col/CM on burn wounds in rats showed improvement in wound closure, and histopathological examination showed that the PVA-Col/CM scaffold accelerated burn wound healing through anti-inflammatory activities, more abundant fibroblast cells, more angiogenesis and collagen deposition, greater re-epithelialization and dermal remodeling of collagen in the wound area. Totally, this study exhibits the potential of PVA-Col/CM as a treatment for burn wounds.
