Abstract:
The mushroom Inonotus hispidus is traditional Chinese medicine, which has been used to treat tumor illness for a long history in China. I. hispidus mushroom mainly grows on five different tree species (Morus alba L., Ulmus macrocarpa Hance, Fraxinus mandshurica Rupr., Ziziphus jujuba Mill., and Malus pumila Mill.), and their fruiting bodies were all separately used in the market. To discuss the anti-tumor mechanism of I. hispidus petroleum ether extract (IPE) on H22 tumor-bearing mice from the point of view of metabonomics. We found that I. hispidus petroleum ether extract (IPE) has significant anti-tumor activity. Moreover, we explored the potential antitumor regulatory pathways and targets of I. hispidus, and performed whole transcriptome and proteome analyses in the H22 tumor-bearing mice model.The combined omics analysis revealed five key genes/proteins, including Lilrb4a, Nrp1, Gzma, Gstt1 and Pdk4, which may play a role in the anti-tumor pathway and were validated.
