Title : Novel QRN substance effect of non small cell lung cancer cells
Keywords: QRN substance, Novel Substance, Non-small cell lung cancer, Novel Therapy
Introduction:Cancer is a scary name even though it is named after an innocent animal, even called a disease. It has sought the door to many different ideas working to experience many other theories and treatment alternatives as a disease. Despite the drugs that are still used in many types of treatment, the only real death that cannot be prevented due to the disease is the end. When the products needed are provided, the old chemical balance is restored, otherwise every cell must continue to do its job, which is to keep the organism alive, even if it is uninterrupted. For this purpose, we have been examining and working on various studies and various sources for 11 years, and we determined the name that enabled us to find this substance as the name of the raw material of the study. Like all scientists, our aim is to wake up to a better world the next day and compete in goodness.
Material-Method:An MTT assay is a colorimetric assay based on assessing the cell metabolic activity. A549 Lung adenocarcinoma cell line was used to see the cytotoxic potential of a new drug for initial screening of apoptosis or necrosis. The formed formazan can be dissolved with dimethyl sulfoxide (DMSO) to give a purple color with characteristic absorption at 540 nm. Intensity of purple color is directly proportional to the cell number and thus indicating the cell viability. We use MTT method evaluate QRN substance effect of A549 non-small cell lung cancer cell line.
Result:After culturing the A549 cell line, the drug was administered for 24, 48, 72 hours. While the number of cells in the control group increased over time, a significant decrease in cell proliferation was observed in the drug(QRN substance)-administered cell groups. In the control and bdH2O groups, the number of A549 cells increased with time. In the QRN administered group, the number of viable cells decreased by 62.8% in 24 hours, 52.6% in 48 hours and 35.8% in 72 hours.