COPD 2021

Elham Harati

Elham Harati, Speaker at Pulmonology Conference
Tehran University of Medical Sciences, Iran (Islamic Republic of)
Title : Effects of viola tricolor flower hydroethanolic extract on lung inflammation in a mouse model of chronic asthma

Abstract:

Asthma is a chronic inflammatory disease of the lungs driven by T cell activation. Viola tricolor L. as a traditional medical herb could suppress activated T lymphocytes and has been used empirically for asthma remedy. In the present study, we investigated the anti-inflammatory effect of Viola tricolor and its underlying mechanism on asthma characteristics induced by ovalbumin (OVA) in mice. BALB/c mice were randomly divided into six groups: normal control, Ovalbumin (OVA) control, OVA mice treated with Viola tricolor (50, 100 and 200 mg/kg) and dexamethasone (3mg/kg). All mice except normal controls were sensitized and challenged with OVA. Asthmatic mice were treated orally in the last 7 days of OVA challenge. The total and differential leukocyte counts, Interleukin (IL)-4 and interferon (IFN)-γ levels in bronchoalveolar lavage fluid (BALF) were determined. H&E staining for lung inflammation was performed. Viola tricolor treatment at 200 mg/kg significantly decreased IL-4 level but did not considerably affect the IFN-γ level. Therefore, it effectively reduced asthma characteristics including infiltration of leukocytes particularly eosinophil and peribronchial inflammation as compared to dexamethasone. However, Viola tricolor at 100 mg/kg had the most prominent inhibitory effect on the IL-4 level and also markedly increased IFN-γ level. As result, it prevented further reduction of inflammatory parameters in this group compared to the Viola tricolor-treated group at 200 mg/kg. Our study demonstrated that Viola tricolor has anti-inflammatory effects via inhibition of Thelper type 2 (Th2) cytokine production and validated its empirical usage in traditional medicine.

Biography:

Dr. Harati studied Physiology at the Tehran University of Medical Sciences, Iran and received her PhD degree in 2018. She is interested in allergic asthma and psychiatric and neurological disease. She is a researcher at Iranian Center of Neurological Research, Neuroscience Institute, Imam Khomeini Hospital, Tehran University of Medical Sciences, Tehran, Iran. She is a reviewer and an editorial board member of several journals. She is a member of the Iranian Asthma and Allergy Association. She is the Invited Assistant Professor of Physiology at Islamic Azad University, Tehran Medical Branch.

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