Title : Effect of photobiomodulation on odontogenic differentiation of human gingival mesenchymal stem cells
Introduction: The use of mesenchymal stem cells (MSCs) in regenerative dentistry is becoming an increasingly vital and interesting area of research. MSCs derived from dental origins have shown great applications due to their multilineage differentiation potential and self-renewal capacity. Gingival MSCs are among the dental MSCs that are characterized by unique immunomodulatory functions in addition to their multipotent differentiation ability. Gingival MSCs are considered to be of a very accessible origin and could be considered as an alternative source of MSCs to be used in regenerative dentistry. Photobiomodulation has been reported to be a promising source to positively affect the MSC's self-renewal and differentiation ability.
Aim: In this study, photobiomodulation was used to investigate its effect on the odontogenic differentiation ability of gingival MSCs.
Methods: Gingival MSCs were collected and characterized by flow cytometry and multilineage differentiation ability. Cells were seeded in 24 well plates for odontogenic differentiation and were irradiated once by laser of wavelength 890nm at energy 3J/cm. Cultures were grown for 14 days after which alkaline phosphatase (ALP) assay, Alizarin red staining, and odontogenic markers by real-time PCR were measured.
Results: ALP and Alizarin red measurements of irradiated cultured cells showed a statistically significant greater mineralization than control samples. Odontogenic markers were also found to be significantly greater in irradiated cells.
Conclusion: The results showed that gingival MSCs responded positively to the photobiomodulation in terms of odontogenic differentiation.
Audience Takes Away Notes:
- Gingival MSCs can be utilized as an accessible source of MSCs
- Photobiomodulation can enhance the odontogenic potential of gingival MSCs
- Further research can be done to detect the effect of different dental materials on MSCs when irradiated with photobiomodulation.