Title : Stem cell homing for regenerative endodontics in nano composite hydrogel patterns
Abstract:
Clinically, functional pulp-like tissue should be capable of producing vascularized and re-innerved tissue that controls the amount of dentin deposition in dental tissue. In this situation, the pulp regenerates close to the healthy pulp. About 50 years ago, Nygaard-Ostby evaluated the hypothesis that blood clots have healing effects in the treatment of damaged tooth pulp. The findings revealed that the blood clot in the pulp tissue of the tooth did not regenerate entirely. Subsequent studies showed that by intentional bleeding into the root canal in immature necrotic teeth, living tissue regenerated, which was associated with neural network sensitivity. Cell homing is an underappreciated approach to tissue regeneration that provides an alternative to cell-delivery-based tooth regeneration and appears to provide a tangible pathway toward clinical translation. Dental stem cells would be a suitable candidate for endogenous stem cells for dental tissue regeneration. Cell-homing approach based on stem cells residing in the oral tissue regenerates new like-pulp with the help of growth factors secreted from the engineered scaffold that can overcome all cell transplantation strategy’s challenges. Hydrogel scaffold can be a suitable substrate for dental stem cells to recruit, proliferate, and differentiate. For example, it was proven that a designed peptide-based hydrogel with bFGF for injection into the root canal could serve as a carrier of different molecules as well as a good substrate for the growth of target tissue cells. It may also be used to combine soft and hard tooth tissue to regenerate endodontics.