Plaque glycolysis and regrowth methods: What it is, why it is important, and how it can help your research and formulation endeavors

Title : Plaque glycolysis and regrowth methods: What it is, why it is important, and how it can help your research and formulation endeavors

Abstract:

Plaque growth and regrowth methods (PGRM) were introduced in the mid-1990s as a creative method of demonstrating antimicrobial efficacy for stannous fluoride (SnF₂) and cetylpyridinium chloride (CPC). While there are variation based on the original published model, the purpose of these are two explore the putative action with respect to either glycolysis or regrowth. While there are many agents that inhibit microbial regrowth, fewer can inhibit glycolysis. As such, those agents – such as CPC – that deliver both benefits are regarded as the most promising. This workshop will share some details of the original published 1995 PGRM model, provide insight into variations of the PGRM model, discuss how PGRM models are complementary to antiplaque and antigingivitis research and testing, and touch on specific commercially available products that may or may not demonstrate regrowth and glycolysis efficacy. In explaining applicability of these models to various agents, we will use insights and results from our glycolysis and automated plaque regrowth (GAPR) model (Karlinsey & Karlinsey. Dent. J. 2024, 12(5), 146; an in vitro model that utilizes harvested human plaque.

Audience take away notes:

• Discuss the two primary outcomes in using PGRM models and the importance of each
• Examine how PGRM models, including the GAPR model, can facilitate your research, testing, or quality control endeavors
• Show how PGRM models might complement other lab-based and clinical evaluations
• Explore how PGRM models might be used to help understand the mechanism of action of a specific putative antiplaque or antigingivitis ‘active’.

Biography:

Dr. Robert L. Karlinsey is a scientist and formulator with almost 20 years’ experience in dental research. He earned a BS in Physics (University of Scranton) and PhD in Chemical Physics (Indiana University), with postdoctoral work in physical chemistry (Indiana University). As a visiting assistant research professor at the Indiana University School of Dentistry, his research led to an impactful encounter with Dr. George Stookey, and shortly thereafter, the formation of Indiana Nanotech, LLC (now known as Custom Dental Formulations, LLC), of which he spearheaded all research and small-scale manufacturing efforts. Dr. Karlinsey is the inventor of Nanotech’s functionalized tricalcium phosphate systems, which continue to help generate over $100MM in global product sales for a major dental manufacturer.  As Principal Investigator, his work has received significant funding through Federal (National Institutes of Health), State (Indiana and Texas), and industry grants and contracts. His research in patented calcium phosphate technologies continue to help generate over $100MM in global product sales. Previously, Dr. Karlinsey has served as Chief Scientific Officer at Therametric Technologies, Inc, and adjunct professor of chemistry at the University of Indianapolis. Dr. Karlinsey addresses clients’ needs in preventive and cosmetic dentistry, including fluoride- and fluoride-free remineralization, antiplaque/gum health, whitening systems, and more. He is actively involved in clinical and laboratory research designs and performance-backed, customized oral care formulation solutions. And, whenever possible, he favors natural, nature-derived, and/or sustainably sourced ingredients.