Title : Formulation and Evaluation of Biodegradable Polymeric Scaffolds containing Mupirocin Microspheres for Improved Wound Healing
Abstract:
Development of a bio-composite using synergistic combination is a promising strategy to address various pathological manifestations of acute and chronic wounds. In the present work, we have combined three materials viz., mupirocin as an antimicrobial drug, mupirocin-chitosan microspheres (Mu-CM) as drug carrier for sustained delivery of drug and PLGA-PEG , as an biodegradable scaffold. Mupirocin microspheres were prepared by emulsion cross linking method. Tannic acid was used for cross linking of chitosan instead of glutaraldehyde. The mupirocin microspheres were found to be spherical by SEM and having mean particle size 3.29µm.The entrapment efficiency of mupirocin microspheres was found to be 81.22% and were able to release drug in sustained manner for more than 5days. PLGA biocomposite scaffolds were prepared using solvent cast/particulate leaching technique and were compared with mupirocin loaded and Mu-CM loaded PLGA scaffolds for various parameters like surface morphology, tensile strength, entrapment efficiency and distribution homogeneity, in vitro drug release, water uptake capacity, cell proliferation on(L929) human fibroblast cell lines, antibacterial activity and stability study, In vivo wound healing efficacy using excision wound model in Wistar Albino rats. The Mupirocin microspheres incorporated PLGA scaffolds showed good in vitro and invivo characteristics in terms of better water uptake, sustained drug availability and antimicrobial activity, faster wound healing. Consequently, the developed formulation with synergistic strategy of combining Mu-CM and PLGA scaffold as a dressing material would be ideal biomaterial in controlling the infection for a longer period and reducing the frequency of application which leads to faster wound healing and cost effective treatment of surface wounds, burns and foot ulcers.
