3rd Edition of International Conference on Tissue Engineering and Regenerative Medicine

August 21-23, 2023

August 21 -23, 2023 | London, UK
TERMC 2020

Victor Allisson da Silva

Victor Allisson da Silva, Speaker at Tissue Engineering Conference
Federal University of ABC, Brazil
Title : Evaluation and characterization of neural integration within polymeric scaffold aiming spinal cord regeneration


Tissue therapy in cases of spinal cord injury is of great importance for the development of treatments aimed to nerve regeneration. One of the main methods is the use of polymeric scaffolds, which are structured biomaterials that promote cell support and stimulate cell differentiation at injured sites. However, cellular responses may vary according to the macro and microstructure of these materials. Regarding macro configurations, one can obtain scaffolds ranging from films, cylinders, tubes,  channels,  and  even  hydrogels.  In  addition,  the  microstructural  conformations  may  be  unique,  allowing  variations  in the mean pore diameter, hydrophilicity, composition and other surface characteristics of the biomaterials. However, these properties should be evaluated in vitro before proceeding to the in vivo application.
Objectives:  The aim of this study was to evaluate and characterize the cytotoxicity and development of spinal cord cells in contact  of polymeric  biomaterials  films  based  in chitosan  (CHI);  poly-L-lactic acid  (PLLA); polycaprolactone  (PCL)  and f ibrous polycaprolactone (PCLf) for spine regeneration.
Methods:  The sterilization method used was ultraviolet (254 nm, 30 minutes) on each side of the biomaterial. With each polymer were performed: a) VERO cell cultures for cytotoxicity analysis, through direct and extract contact, in addition to MTT tests; b) mixed primary cultures of spinal cord of neonatal rats with 0-3 days postnatal (P0-P3) for evaluation of fixation, cytotoxicity,  differentiation,  dendritic  branching  and  connectivity  through  light  microscopy  and  transmission  electronics.  All experiments were carried out according to protocol 4509160816 approved by CEUA/UFABC.
Results and Conclusions:  Based  on  the  cell  viability  assays  (MTT  test),  it  was  observed  that  the  PCL  (1.067±0.013),  PCLf (1.089±0.028), PLLA (1.169±0.066) and CHI (1.068±0.014) composites obtained similar results to the negative control (1.000±0.042) , where the cells developed properly within 24 hours after incubation, demonstrating no cytotoxicity. With the result of non-cytotoxicity of the polymers, the cells of the spinal cord of neonates were incubated on them, allowing observations  on  the  differentiation  and  development  of  this  culture  in  direct  contact  with  the  biomaterials.  In  comparison  to the negative control, the biomaterials resulted in similar morphologically cells. With all of this in mind, we suggests that the polymeric films of PCL, PCLf, PLLA and CHI allow adhesion and correct differentiation for both Vero cell lines and spinal cord cells. Thus, all the information suggests that the use of scaffolds may be a great alternative for spinal regeneration. Key-words:  cellular viability, spinal cord injury, scaffolds, biomaterial.


Biomedical engineer with an emphasis on tissue engineering from Universidade Federal do ABC (UFABC) - Brazil. He has been working for more than 5 years with regenerative medicine applied to the spinal cord, participating in several projects and clinical studies with national and international groups, working with bioprinting techniques, differentiation of stem cells to neuronal lineages and biomaterials applications as regenerative  tools. During his education he had the opportunity to study his area of interest in Canada (University of Victoria - 2018) and South Korea (University of Seoul - 2019)