3rd Edition of International Conference on Tissue Engineering and Regenerative Medicine

August 21-23, 2023

August 21 -23, 2023 | London, UK
TERMC 2020

Inna Bulygina

Inna Bulygina, Speaker at Regenerative Medicine Conference
National University of Science and Technology "MISiS", Russian Federation
Title : Method of mimicking mammalian trabecular bone architecture for bone implants manufacturing

Abstract:

A biomimetics is a promising approach allowing to create materials with new specific properties mimicking natural structures. This study demonstrates the direct replication of trabecular bone architecture. The biomimetic scaffold was constructed using chemically inert and biocompatible ultra-high molecular weight polyethylene (UHMWPE) polymer. The main issue associated with UHMWPE is a complex processing, a time-consuming procedure which makes it difficult to prepare a 3D-printed sample. Thus  present study  proposes  a feasible  method  for  fabricating an implant having  native trabecular  bone architecture. The scanning electron microscopy and computer tomography revealed the samples were composed of macro and microstructures which were found very similar to native bone architecture. The pore size of the UHMWPE was found to be ranging from 500 µm to 700 µm whereas the average thickness of the wall between pores was noticed as 80 µm. This value was observed to be very small as compared to that of bone (200 µm). Moreover, the nanorelief that existed on the surface of the sample can be beneficial for cell proliferation. These structural features indicate the efficiency of this method which can find its potential application in the biomedical field. The observed topography combined with etched surface resulted in enhanced adhesion and proliferation of cells. Multipotent mesenchymal stromal cells (MMSCs) were incubated with the UHMWPE scaffold. After 4 hours 40 % of cells adhered to its surface. Further, when the co-incubation time was increased to 48 hours the proliferation rate grew and reached 75 %. The cytocompatibility study indicated that the sample provided the necessary environment for the adhesion and proliferation of MMSCs.

Biography:

Ms. Inna Bulygina studied Nanomaterials at the National University of Science and Technology “MISiS” (NUST “MISiS”), Moscow, Russia. Being  an  undergraduate,  she  joined  the  research  group  of  Professor  S.  Kaloshkin  at  the  Center  for  Composite  Materials,  NUST  “MISiS”, Russia. Bachelor’s thesis topic covered methods of UHMWPE bone implants with native trabecular bone structure manufacturing. Currently, she is a Master’s degree student at Materials Science under the supervision of Professor Fedor Senatov at the NUST “MISiS”. She is working on the cellulose hydrogels and PLA-based scaffolds. She has published several research articles in SCOPUS indexed Journals.

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