Title : Cytotoxicity and cell viability of two bioactive root canal sealer, mineral trioxide aggregate and bioroot root canal sealers: An in vitro study
Abstract:
Aim: The aim of this study is to perform a comparative assessment of cytotoxicity and cell viability of two bioactive root canal sealers (RCS), mineral trioxide aggregate (MTA), and BioRoot-RCS.
Materials and methods: Mineral trioxide aggregate (MTA) and BioRoot-RCS are based on calcium silicate composition, MTA sealer (Brasselers, Savannah, Georgia, United States of America) is a premixed injectable material based on calcium silicate, BioRoot-RCS (Septodont, Saint-Maur des-Fossés, France).
Powder: Tricalcium silicate zirconium oxide and povidine. Liquid, water, calcium chloride, and polycarboxylate. Cytotoxicity of these two bioactive sealers was assessed by Metabolic Activity Assay (MTT) reduction test, where human gingival fibroblast (HGF) where used; this method enables determination of cell viability and proliferation on the basis of mitochondrial activity of succinate dehydrogenase, days 1–7 were studied, while the cell viability (survival percentage) was measured on 1, 3, 5, and 7 days.
Result: The result between the two BioRoot-RCS showed higher cell viability or fibroblastic survival percentage and low cytotoxicity for BioRoot RCS in comparison with MTA-RCS.
Conclusion: Mineral trioxide aggregate (MTA) and BioRoot-RCS both show moderate cytotoxicity. However, BioRoot-RCS is considered the most compatible root canal sealer showing a higher percentage of cell viability as compared to MTA.
Audience Take Away:
- Cytotoxicity and cell viability of two bioactive root canal sealers (RCS), mineral trioxide aggregate (MTA), and BioRoot-RCS.
- Implementation of new sealers in day to day practice.
- Further research is required for new formulations and safety of sealers.
- The article provides cytotoxic effect and comparison between two sealers, which helps the practitioner to understand its post operative effect after extrusion beyond the apex.
- Further research is required.
- With reference to the conclusions in the present study further comparison and research on recent bioactive sealer can be done before their implementation in clinical practice.
