Title : Nanostructured materials used for the fabrication of an AcHE biosensor for pesticide detection
Abstract:
Nowadays agriculture cannot be conceived without the pesticides usage to protect the crops. The pesticide term includes chemicals substances intended for plant growth factor, defoliant, desiccant or agent for thinning fruit or preventing the premature fall of fruit, substances applied to crops either before or after harvest for preventing, destroying or controlling any pest. Unfortunately, the pesticides pollute a large palette from environment: plants, organisms, soil and water. Using monitoring tools, like chemical biosensors, the use of pesticides can be put under control. Microelectronics offers some convenient transducers. Borrowing micro-technological processes, the enzymatic biosensors can be easily integrated onto the Silicon wafers. This paper proposes a biosensor for paraoxon pesticide detection, as a small piece from a decontamination soil technology plan. The paper reveals the pesticide detection principle, by paraoxon hydrolysis assisted by the Acetyl-cholinesterase enzyme, as key receptor. The key process is must find a compatible enzymatic membrane deposition on the Si-wafers. A good entrapping occurs if the enzyme is captured on a nano-porous thin layer by adsorption combined with other cross-link methods. A main advantage of the Si nano-porous layer directly grown on a wafer consists in strong anchoring to the Si-substrate. Converting p-type Si in Si-porous by anodization, is a solution. Then, some technological steps, with tests and microscopy analysis are presented. Finally, the preliminary tests of the developed biosensor with the AcHE enzyme immobilized onto the Si-porous layer, are discussed.
Audience Take Away:
- How a biosensor of pesticides using AcHE enzyme and Si-substrate can be co-integrated.
- Si nano-porous structure action
- The presented technique can be use to expand the research field of environmental biosensors
- The issue provide a practical solution to co-integrate biomaterials near electronic devices
- The presented solutions could improve the accuracy of a biosensor design.
