Title : Luminescence insights to binding interactions of graphene oxide with pepsin: A spectroscopic and molecular docking approach
Abstract:
Graphene oxide (GO) is wonder material yet a controversial in the biomedical field irrespective of its wide applications in drug delivery, wound healing, biosensors, etc. The interaction of GO with proteins is complex and depends basically upon the properties of GO. Pepsin is the gastric enzyme responsible for protein digestion. Pepsin is involved in the pathophysiology of several disease therefore the interaction of GO with pepsin may reveal insightful facts. In present study the interaction study of GO with pepsin is performed by various spectroscopic techniques as well molecular docking studies. The UV-Vis study revealed the hypsochromic shift indicative of formation of ground state complex. The fluorescence study revealed both static and dynamic nature of quenching. The thermodynamics studies further revealed the spontaneous nature of the interaction. Further the enzyme activity of pepsin was found to deceased significantly. The molecular docking results supports all the studies revealing that hydrogen bonding and pi-pi interaction dominates the interaction between GO and pepsin.
Audience Take Away Notes:
- Formulation development for gastric delivery of GO
- Knowledge of Protein-ligand interaction with new drugs
- Secondary structure analysis of protein in presence of nanomaterial
- Protein corona analysis
