Title : Synthesis and physicochemical characterization of tannic acid encapsulated BSA nanoparticles: From structural analysis to bioactivity assessment for enhanced drug delivery
Abstract:
BSA-NPs offer a beneficial strategy for targeted drug delivery. These NPs improve the drug effectiveness by increasing its availability and distribution. At the same time, these NPs reduce the body tendency to develop resistance towards drugs. Tannic acid (TA), a naturally-occurring polyphenolic compound, exhibits strong antioxidant property and it has been tested for its anticancer property against different types of cancer. In present study, BSA nanoparticles encapsulated Tannic acids (TA-BSA-NPs) were synthesized and characterized using FE-SEM, FT-IR and UV-Vis spectroscopy. The TA-BSA nanoparticles, about 300 nm in diameter with a surface zeta potential of ∼-37 mV, and encapsulation efficiency of 78%, have shown sustained release of TA up to 75% during in vitro studies. Cellular uptake investigations demonstrated that more than 80% of MDA-MB-231 cells i.e., human breast carcinoma cell line uptake TA-BSA NPs. The present study also narrows down the research gap by investigating the conformational changes in BSA-NPs after TA encapsulation via FT- IR. The critical findings indicate % increase in β-sheet content 56 % in BSA NPs and it decreased by 33.7 % for TA-BSA NPs. In summary, these findings suggested significant improvement in TA delivery after encapsulation in sustained manner. The outcomes of the chemical morphology analysis and in vitro release experiments suggest that these nanoparticles hold promise as vehicles for delivering drugs in biological settings where a release of TA is needed.
Audience Take Away Notes:
- My lecture will specifically help researchers in the field of biochemistry to design new drug delivery agents and how to test their efficacy
- My research is noble as I have explained for the first time the changes in the secondary structure of BSA when BSA changes to BSA nanoparticle
- In our study we have optimized the procedure for interlinking tannic acid with BSA nanoparticle
- finally we have tested the drug delivery efficacy of drug loaded BSA nanoparticle
