Fluorescent NH2-mil-88(Fe)-stabilized Ptnps nanozyme for dual-channel “off-on” simultaneous and visual detection of glyphosate and Pb2+ in agri-environments and foodstuffs

Title : Fluorescent NH2-mil-88(Fe)-stabilized Ptnps nanozyme for dual-channel “off-on” simultaneous and visual detection of glyphosate and Pb2+ in agri-environments and foodstuffs

Abstract:

Trace levels of pesticides and heavy metals in the environment and foodstuffs represent a substantial threat to human health. This study presented a bifunctional NH₂-MIL-88(Fe)@Pt nanozyme possessing superior peroxidase-mimicking activity and fluorescence properties, further establishing a dual-channel "OFF-ON" sensing platform for the simultaneous visual detection of glyphosate (GLY) and Pb²⁺. In the "OFF-ON" mechanism, cysteamine (CA)-mediated catalytic suppression of TMB oxidation was reversed through specific Pb²⁺ chelation, enabling quantitative Pb²⁺ detection with a limit of detection (LOD) of 0.0049 μg/mL (0.024 μM). The abundant –NH₂ groups on NH₂-MIL-88(Fe)@Pt induced fluorescence quenching via Cu²⁺-triggered Lewis acid-base coordination. The preferential binding of Cu²⁺ to GLY restored fluorescence, facilitating quantitative detection of GLY (LOD: 0.0082 μg/mL (0.048 μM)). Based on these principles, a hydrogel-based NH₂-MIL-88(Fe)@Pt nanozyme platform incorporating smartphone analysis was developed to achieve semi-quantitative visual detection of GLY and Pb²⁺, demonstrating significant potential for efficient contaminant screening in agri-environments and foodstuffs.

Biography:

Dr. Li is studying Food Science and Engineering at Tianjin University of Science and Technology and obtained a Master of Medicine degree in 2024. Subsequently, she joined the research team of Professor Pan Mingfei at the School of Food Science and Engineering of Tianjin University of Science and Technology to pursue a doctoral degree. Her research direction is the precise and rapid analysis and detection of trace toxic and harmful substances in Food safety. She has published more than 8 research articles in SCI journals such as Trends in Food Science and Technology, Chemical Engineering Journal, Food Chemistry and Analytica Chimica Acta.