Title : Plant_mediated synthesis TiO2nanoparticles: Evalution of antimicrobial and anticancer activities
Abstract:
Green nanotechnology provides an eco-conscious approach for nanoparticle synthesis compared to conventional chemical methods. In the present work, titanium dioxide (TiO₂) nanoparticles were fabricated using the leaf extract of Ajuga macrosperma, a medicinal plant rich in phytochemicals. The biomolecules present in the extract functioned as natural reducing and stabilizing agents, facilitating a sustainable synthesis pathway. Characterization techniques including UV–Vis spectroscopy, FTIR, XRD, SEM, and EDX confirmed the nanoscale size, crystalline structure, and interaction with plant metabolites. The biosynthesized nanoparticles exhibited pronounced antibacterial activity against Staphylococcus aureus, Bacillus subtilis, Escherichia coli, and Pseudomonas aeruginosa. Furthermore, cytotoxicity studies using the MTT assay on A549 (lung), HeLa (cervical), MCF-7 (breast), and PC-3 (prostate) cell lines revealed dose-dependent anticancer effects mediated by reactive oxygen species (ROS) generation and apoptosis induction. These results suggest that Ajuga macrosperma–derived TiO₂ nanoparticles are not only environmentally friendly but also promising candidates for biomedical applications such as antimicrobial therapy and cancer treatment.
Keywords: Green Synthesis, AjugaMacrosperma, Characterization, Antimicrobial Activity And Anticancer Activity.
