2nd Edition of International Conference on Tissue Engineering and Regenerative Medicine

September 16-17, 2022 (Online Event)

September 16 -17, 2022 | Online Event
TERMC 2021

Carbon nanotube-reinforced polycaprolactone for 3D bioprinting and tissue engineering

Adam Mieloch, Speaker at Tissue Engineering Conferences
Adam Mickiewicz University, Poland
Title : Carbon nanotube-reinforced polycaprolactone for 3D bioprinting and tissue engineering

Abstract:

3D bioprinting is a rapidly growing field of tissue engineering. Currently, one of the main obstacles is the scarcity of biomaterials tailored for particular use cases. This study is focused on exploring thermoplastic material – polycaprolactone, reinforced with multiwalled (MWCNT) and bamboo (BCNT) type of carbon nanotubes, for use in tissue engineering. Toxicity of various nanomaterials, including carbon nanotubes is still a subject of ongoing debate. The added complexity of hybrid biomaterials necessitates further studies, extending beyond properties of a single constituent, taking into account various interactions between given materials. In the study,  mechanical, structural, optical and biological evaluations of the materials were performed, including: nanoindentation, rheology studies, differential scanning calorimetry, scanning electron microscopy, confocal microscopy, proliferation, and cytotoxicity assays. Human normal chondrocytes were utilized for biological studies. Carbon nanotubes were added in concentrations ranging from 0.005% to 0.2% w/w. Cellink BioX printer with thermoplastic printhead was utilized. Preliminary results indicate that both the concentration and type of carbon nanotubes used significantly affect the mechanical properties and biocompatibility of the scaffold 3D bioprinted with PCL/CNT composite. The study aims to find a link between material`s crystallinity, surface topography, mechanical properties, and biocompatibility, necessary for furthering our understanding of thermoplastic biomaterials in tissue engineering, and 3D bioprinting. This work tries to navigate through the issues inherent for composite bionanomaterials, proposing analytical techniques helpful in evaluation of the materials` properties, crucial from the standpoint of 3D bioprinting and tissue engineering.   

Biography:

Adam Mieloch is a PhD student at the Adam Mickiewicz University in Poznan, Poland, at the Faculty of Chemistry. He graduated as Ms in 2015 in Biotechnology He currently works as a researcher at the Laboratory of Applied Biotechnology, chaired by prof. Jakub Rybka. Adam Mieloch is currently employed as researcher in three projects, two of which are focused on 3D bioprinting, tissue engineering and biomaterials in meniscus regeneration. The third one aims at the development of a novel immunodiagnostic tool for COVID-19. He co-authored several research papers in international journals and a patent application.

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