Hemolytic anemia (HA) often attacks dogs and is challenging to treat. HA could be an autoimmune disorder. Therefore it needs an animal model of hemolytic anaemia. For making HA condition, mimicry materials such as blood from allograft or xenograft are used to induce autoimmune to against the blood. The purpose of the research was to examine the comparative effects of allograft and xenograft blood to induce HA in mice. The results were considered based on complete blood count (RBC, WBC, Hb, PCV), blood chemistry (TPP, bilirubin, globulin, BUN, and creatinine), blood smear description, CD4+ and CD8+ of the spleen, and liver, spleen, kidney histopathology. There were three groups, namely negative control, allograft blood induction, xenograft blood induction. Allograft blood was obtained from other mice from a different source. Xenograft blood was obtained from cat blood. The blood was injected intraperitoneally (i.p) 0.2 ml 5 times per week until seven weeks. The results showed that both types of blood induced hemolytic anaemia with a different type and impacted liver, spleen and kidney tissue. Xenograft blood group has a higher (p<0.05) WBC, bilirubin, BUN
level, number of CD4+ and CD8+ cells of spleen and lower (p<0.05), albumin and creatinine level than that of allograft blood group than of allograft. Both allograft and xenograft blood induce the tissue destruction of the spleen, liver and kidney. Conclusions of the research were the allograft blood caused fast hemolytic anemia and the xenograft blood induced the delayed hemolytic anaemia.