2nd Edition of International Conference on Tissue Engineering and Regenerative Medicine

September 16-17, 2022 (Online Event)

September 16 -17, 2022 | Online Event
TERMC 2021

Curvature as a guidance cue for single cell migration and epithelium morphogenesis

Laurent Pieuchot, Speaker at Tissue Engineering Congress
Universite de Haute-Alsace, France
Title : Curvature as a guidance cue for single cell migration and epithelium morphogenesis


Cells can migrate individually between tissues and organs or collectively in tightly or loosely associated groups. In both cases, cell migration is regulated by extracellular cues of very different natures. Here I will describe a cellular mechanism called curvotaxis that enables individual cells and growing epithelial monolayers to sense and respond to curvature variations from the substrate on which they adhere. Live imaging combined with functional analysis and in silico modeling shows that curvotaxis relies on a dynamic interplay between the nucleus and the cytoskeleton - the nucleus acting as a negative curvature magnet that leads the migrating cell towards concave curvatures. Curvature affects also focal adhesions organization and dynamics, nuclear shape and gene expression. Growing epithelial colonies can also respond to curvature by a process involving both curvotaxis in the leading edge where cells are loosely associated, and oriented cell divisions in the more mature parts of the colony. Altogether, this work identifies cell-scale curvature as an essential physical cue that can guide both single and collective cell migration processes. Potential applications in tissue engineering and biomaterial design will be discussed.


Dr. Laurent Pieuchot is permanent researcher (CRCN) at the CNRS. After a Ph.D. in cellular and molecular biology in 2009, Laurent moved to the National University of Singapore to study fundamental mechanisms that govern cell growth and differentiation. He worked on a variety of topics, ranging from organelle biogenesis in mammalian cells to the systems biology of multicellular fungi. He obtained a permanent CNRS position (CR1) in 2016 and joined the IS2M, Mulhouse to develop approaches combining cell biology and material sciences. He focuses his current research on the role of topographical curvature in various cell biology processes.