Title : Combination of tofu okara and etawa goat milk prevented inflammation and bone loss in the menopausal rat model
Abstract:
Osteoporosis is a disorder characterized by bone mass loss due to reduced calcium absorption. The menopause stage induces downregulation of calcium absorption in the intestine which caused low estrogen levels. One of the sources of estrogen could be phytoestrogen from Tofu. Exogenous calcium would be from milk like goat milk. The research proposed to examine the impact of the combination of Tofu okara and Etawa goat milk for inflammation and bone loss prevention in the menopausal rat model. The twenty female Wistar Rats, 8-12 weeks of age, were divided 5 groups: 1) negative control as normal Rats; 2) positive control as ovariectomized rats; 3) ovariectomized rats with treatment tofu okara 0.159 g/kg BW PO; 3) ovariectomized rats with treatment Etawa goat milk 0.3 mL; 5) ovariectomized rats with treatment combination Tofu okara and Etawa goat milk. The treatment was conducted one day after ovariectomy which was once daily until 30 days. The mandibular bone and femur bone were collected after the treatment was completed and then processed to tissue slide. The result showed that bone matrix and Bone Morphogenetic Protein 2 (BMP-2) expression was bigger significantly p<0.05 in group 5 compared with other groups. In addition, Tumor Necrosis Factor (TNF-α ) expression and osteoclast count were at the lowest levels in group 5. The combination of Tofu okara as a phytoestrogen which is estrogen-like and Etawa goat milk as a calcium source prevents the bad effect of menopause in a rat model. The research concluded that the combination of Tofu okara and Etawa goat milk prevented bone loss and inflammation in the menopausal rat model.
