Title : Effect of nanotechnologically treated physiological saline solution on clinical and physiological parameters of preserved donor erythrocytes during the stages of their storage under hypothermic conditions
Abstract:
Background: The benefits gained by improved RBCs component quality should more than justify any real or perceived inconvenience to the blood services in implementing adjustments to their processing procedures or additional processing costs of the introduction of new generation RBCs additive solutions. Recently the FDA has tightened and increased the assessment and acceptance criteria making it potentially more difficult and expensive to bring new RBCs storage systems to market. The researches has proved that now of magnetite nanoparticles are able not only to considerably reduce hemolysis, and thereby prolong storage time of the blood’s heparinized, influence on activity of adenosinetriphosphateses of erythrocytes, regulated transmembrane exchange, but also to extracorporeally influence on cellular apoptosis. The above was the basis for the choice of the theme of this study, devoted to the learning of the use of nanotechnology to correct the functional activity of red blood cells at the storage stages at a positive temperature.
The main purpose of the first stage of the study is to develop a simple and practical method of additive modernization of preservation solutions that does not violate the compliance requirements, improves the quality, efficiency and safety transfusion of red blood cells.
Object of research: red blood cells (RBCs) into bags containing anticoagulant citrate, nutrient phosphate and dextrose (CPD); red blood cells (RBCs) into bags containing anticoagulant citrate, nutrient phosphate, dextrose and adenine (CPDA-1).
Materials and methods: magnetite of nanoparticles (ICNB); saline solution of NaCl; MR-tomography; visual analysis of hemolysis; controlled by photometric method hemolysis; microscopic method; Panchenkov's method; pH metric.
Results: It was established that saline NaCl which had previously been processed by magnetite nanoparticles (ICNB) had a marked membrane-stabilizing effect, inhibits haemolysis and increasing the sedimentation stability of preserved RBCs. The complex analysis of the obtained data allowed to determine the primary mechanisms effect of the saline NaCl which had previously been processed by ICNB on the preserved RBCs. The proposed method of additive modernization of preserved RBCs was adapted to the production process. The optimisation results were obtained in creating a simple and practical method of additive modernization of preservation solution that does not violate the compliance requirements, improves the quality, efficiency and safety transfusion of RBCs. The effect of haemolysis inhibition by the method of additive modernization of preservation solutions, that adapted to the manufacture process on day 35 of the study is shown in Fig.1.
