Title : Fat extract improves fat graft survival via proangiogenic, anti-apoptotic and pro proliferative activities
Abstract:
Nanofat has been reported to enhance fat graft survival by promoting neovascularization. We previously introduced fat extract (FE), a cell-free component derived from nanofat, and demonstrated its proangiogenic potential. This study aimed to investigate the effect of FE and its combined application with nanofat on fat graft survival. In the first animal study, macrofat from lipoaspirate was co-transplanted with either FE or nanofat into nude mice. The grafts were evaluated at 2, 4 and 12 weeks post-transplantation. The nanofat- and FE-treated groups showed significantly higher fat graft weights compared to the control group. Moreover, these groups exhibited improved fat integrity, increased viable adipocytes, enhanced CD31-positive blood vessels, reduced apoptotic cells, and increased Ki67-positive proliferating cells. In the second animal study, nude mice received a mixture of macrofat and nanofat, followed by intra-graft injections of FE at days 1, 7, 14, 21 and 28 post-transplantation. After 12 weeks, the fat grafts in the nanofat+FE group had significantly higher weights than the control group. In vitro, FE showed proangiogenic effects on HUVECs, anti-apoptotic effects on fat tissue under hypoxic conditions and an ability to promote ADSC proliferation and maintain their multiple differentiation capacity. Our findings indicate that FE can enhance fat graft survival via proangiogenic, anti-apoptotic, and pro-proliferative effects on ADSCs. FE plus nanofat-assisted fat grafting represents a promising approach in clinical applications.
Audience Take Away:
- Fat extract (FE), a cell-free component derived from nanofat, has demonstrated proangiogenic, anti-apoptotic, and pro-proliferative potential.
- FE-assisted fat grafting improved the survival and quality of fat grafts.
- FE and nanofat could work synergistically to enhance fat graft survival.
- The combination of FE and nanofat in assisted fat grafting holds promise for clinical applications, offering the benefits of both nanofat's high efficiency and FE's advantages of long-term storage and multiple uses.
