Title : Rat’s subcutaneous response toward decellularized goat skin and heterologous Platelet-Rich Plasma (PRP)
Abstract:
The decellularized tissue is famous for grafts that can be a combined growth factor. One tissue graft is dermal matrix-derived animal skin, and the source of the cocktail growth factor is platelet-rich plasma. The study developed a dermal matrix from a goat that combined with heterologous PRP. The research proposed examining the response of rats’ subcutaneous tissue toward decellularized goat skin mixed with PRP. The parameters were extracellular matrix, histopathology features, fibroblast cell count, inflammation cell count, interleukin (IL) 10, 6, 1β, and TNF-α. As many as twenty Wistar male rats, 150-200 grams, were separated into four groups: control group, decellularized goat skin group, PRP group, and mix of decellularized goat skin and PRP group. Decellularization for goat skin used Sodium Dodecyl Sulphate (SDS) solution and PRP preparation from rat’s blood heterologous. The biomaterial based on each group was implanted inside the rats subcutaneously for 14 days. On Day 15th, the subcutaneous tissue-implanted area was collected in NBF solution and continued for tissue processing. The results highlighted that a combination of decellularized goat skin and heterologous PRP reduced IL-10, IL-6, and IL-1β expression. Otherwise, it affected the elevation of fibroblast cells and TNF-α expression. There was good integration scaffold into skin based on histopathology and extracellular matrix features of the combination group, although there was no difference in inflammation cell number among biomaterial implant groups. The conclusion was that the combination of decellularized goat skin and PRP showed good biocompatibility to fasten the integration of the dermal scaffold matrix with host tissue. The decellularized goat skin can be considered for xeno-dermal graft combining heterologous PRP for better results than implanting each material lonely.
Audience Take Away:
- The audience will obtain benefits to learn about decellularized goat skin as candidates of dermal matrix combined PRP
- The industry of pharmacy will receive insight material for dermal graft from goat skin
- The audience will learn the fruitful advantage of the subcutaneous response of rats toward dermal grafts (as combining decellularized goat skin and PRP)
