HYBRID EVENT: You can participate in person at Madrid, Spain or Virtually from your home or work.

4th Edition of International Conference on Tissue Engineering and Regenerative Medicine

September 02-04, 2024

September 02 -04, 2024 | Madrid, Spain
TERMC 2023

Sandeep Shrivastava

Sandeep Shrivastava, Speaker at Tissue Engineering Conferences
Datta Meghe Institute of Medical Sciences, India
Title : Sandeep’s Procedure for Induction of Neo-angiogenesis (SPIN) for management of necrosis in tissue & impending gangrenes


After high-velocity trauma, de-vascularization leads to the death of vital structures including Bone, Tendons, Muscles and loose Skin flaps. Open fractures associated with prolonged exposure of the underlying tissue and the presence of infection are critical factors, leading to further damage. Such tissues undergo physical changes including blackening and tend to peel off.  The bones tend to sequestrate. A vicious cycle of necrosis, infection and more tissue losses sets in. The reversal of vascular viability in these tissues is ordinarily not possible with current treatment. A ray of hope is evolving through propagating “Proliferative healing” by regenerative products such as Platelet Rich Plasma (PRP). The basis of This proliferative healing is induced neo-angiogenesis. We have developed a Technique of inducing neo-angiogenesis and reverting these changes. The technique consists of infiltrating the tissues with platelet Rich Plasma I a standardized protocol. The PRP is a simulant & trigger for proliferative healing leading to the salvage of such critical near-dead tissues. We present case series where in “Gangrene/ impending Gangrene reversals” have been achieved in conditions such as severe necrosis after Compartment syndrome, Diabetic gangrenes and Complex wounds. This study has been conducted over the last 1 decade, since 2009. gradually evolving a therapeutic protocol which is a game changer. The safety, efficacy and standardization towards the form, method, doses and duration for PRP treatment and results is presented. We conclude, “Re-inducing Viability through induction of Neo-Angiogenesis in such near dead tissues is possible through Platelet Rich Plasma by SPIN technique and it triggers the biologic environment conducive to Milieu for their survival and regeneration. 
Keywords: PRP; Gangrene; Neo-Angiogenesis, SPIN technique.


Dr. Sandeep Shrivastava is MS, DNB, PhD. He is Director-Professor of Orthopedics at J.N. Medical College, Datta Meghe Institute of Medical Sciences, (DU) Wardha. Maharashtra. India. He is Group CEO, Meghe Group of Hospitals; Director, Centre of Regenerative Medicine; and Ex DEAN, J.N. Medical College.  He has 76 Indexed Publications, 4 books, 15 copyrights. He has pioneered the wound management with PRP, by developing the clinical protocol of “Sandeep’s Technique” for Assisted Regeneration of Skin (STARS Therapy). His work is widely published and presented across the Globe. He is the author of book – The Illustrative guide on Platelet Rich Plasma