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4th Edition of International Conference on Tissue Engineering and Regenerative Medicine

September 19-21 | Rome, Italy

September 19 -21, 2024 | Rome, Italy
TERMC 2023

Tingting Li

Tingting Li, Speaker at Regenerative Medicine Conferences
Henan Provincial People's Hospital, China
Title : Single-cell RNA-seq reveals the transcriptome differences of spermatogonial stem cells between pre-pubertal and adult buffalo

Abstract:

Aging leads to a progressive decline in homeostasis and regenerative function, including age-related degeneration of stem cell function. In order to investigate whether there were differences in the functions of spermatogonial stem cells(SSCs) at different periods, magnetic activated cell sorting(MACS) was used to separate buffalo spermatogonial stem cell-like cells (SSC-like cells) from pre-pubertal buffalo (PUB) and adult buffalo (ADU), which were used to constructing the transcription maps of buffalo SSC-like cells by single-cell transcriptomic technology. In total 21803 genes from PUB and ADU SSC-like cells identified by Single-cell RNA-seq, among which 10431 were expressed in PUB and ADU SSC-like cells, 9911 were expressed in only ADU SSC-like cells, and 1461 were expressed in only PUB SSC-like cells. GO analysis showed that compared with adult buffalo SSC-like cells, the up-regulated genes in pre-pubertal buffalo SSC-like cells were mainly involved in lipid metabolism, cell surface receptor signaling pathways, and biological processes related to migration. The down-regulated genes were mainly involved in the biological processes related to the meiosis cycle, the meiosis cell cycle and the production of male gametes. Subsequently, through the combination with KEGG analysis, compared with adult buffalo SSC-like cells, the up-regulated expression genes of pre-pubertal buffalo SSC-like cells were mainly involved in the signaling pathways related to the self-renewal of stem cells, while the down-regulated expression genes were mainly involved in the oocyte meiosis pathway, the biosynthesis pathway of secondary metabolites and the insulin signaling pathway. In conclusion, our data provide detailed information on the mRNA transcriptome of PUB and ADU SSC-like cells, revealing differences in function of SSC-like cells at different periods, and providing new evidence for stem cell aging.

Audience Take Away: 

  • Through single-cell sequencing, we found that the number of genes with significantly different expression levels of prepubertal buffalo compared to adult buffalo SSC-like cells totaled 1,419 (fold difference> 2; P <0.05), of which there were 274 significantly up-regulated differential genes, meanwhile, 1145 differentially down-regulated genes. 
  • We performed GO analysis on these differential genes and found that the biological processes involved in the up-regulated expression of SSC-like cells inPUB were mainly lipid metabolism, cell surface receptor signaling pathways, migration processes and other biological processes related to the maintenance of stem cell self-renewal status and stem cell migration. The down-regulated genes were mainly involved in the biological processes related to the formation and differentiation of late gametes, such as the meiosis cycle, the meiosis cell cycle and the generation of male gametes. 
  • We performed KEGG analysis on these differential genes and enriched 151 signaling pathways which may be closely related to the regulatory processes of buffalo SSCs, such as self-renewal, proliferation and migration, and key genes corresponding to these pathways.

Biography:

Dr. Tingting Li studied at the College of Animal Science and Technology of Guangxi University from 2013 and received her doctorate in 2018, and then worked at Henan Provincial People's Hospital.

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