Title : Ultra-high throughput multiphoton 3D bio-printer
Abstract:
Fast development in the field of 3D bio-printing showed immense promise in the capability to produce fully functional organs for in vitro testing or even in vivo implantation. However, while there is an abundance of methodologies that can be employed in this role, the challenge of combining vasculature-enabling micro-resolution with high throughput persists. Therefore, in this presentation, a promising technique of using femtosecond laser in tandem with spatial beam shaping for ultra-high throughput high-precision bioprinting will be presented. Technical aspects and general configuration of such setup are provided. We show that with such a combination sub-1 µm printing resolution can be maintained while offering throughput up to ~several cm3 /h. Furthermore, as it is based on nonlinear absorption, we demonstrate that it has no inherently negative impact on cells present in bio-ink during the printing process. Additionally, we stress that this technology is not inherently limited to only bio-inks and similar materials, allowing the employment of basically any photosensitive resin, including bio-inert hybrid organic-inorganic photopolymers and elastomers. We use this capability to produce a huge array of different bio-oriented 3D structures, such as scaffolds, microfluidic devices, lab-on-chip structures, and stents for various-sized vasculature. With each demonstration, we highlight how multiphoton-based ultra-high throughput femtosecond 3D printing can act as a critical enabler going from laboratory-level experimentation to wider implementation of additive 3D manufacturing in medicine. Details on experimental considerations as well as possible further developments in the field are also provided with emphasis being placed on practical aspects arising from specifically bio-fabrication and tissue regeneration.
Audience Take Away:
- The presentation will show how femtosecond laser-based multiphoton 3D bioprinting can be utilized for biofabrication and organ printing
- The advantages of using optical non-linear absorption for tissue fabrication will be highlighted
- How to increase fabrication throughput by a factor of 10-1000 times in comparison to other similar optical 3D printing techniques without sacrificing ~1 µm resolution by employing spatial beam shaping will be explained
- Details and considerations on materials suitable for such a 3D fabrication method will be shared, steering experts of relevant fields in their research.
