Title : Self-assembled bioactive protein/HA/CUR-based amyloidogenic nanohydrogel dressing for rapid infected diabetic wound healing via enhanced angiogenesis and anti-inflammation
Abstract:
Infected diabetic wounds present a significant clinical challenge due to impaired healing, persistent inflammation, and heightened risk of infection. In this study, we report a novel self-assembled hybrid nanohydrogel composed of Bovine Serum Albumin (BSA)-based amyloid fibrils, hyaluronic acid (HA), and the natural anti-inflammatory agent curcumin, designed as a 3D-printed dressing for effective treatment of infected diabetic wounds. The incorporation of curcumin enhances the antimicrobial and antioxidant properties of the hydrogel, while HA contributes to moisture retention, angiogenesis, and anti-inflammatory response. BSA amyloid fibrils offer structural integrity and self-assembly behavior, forming a mechanically stable yet tunable scaffold. The resultant BSA–HA–Curcumin hydrogel demonstrates excellent water retention, shear-thinning behavior, and biocompatibility, providing a moist healing microenvironment. In vitro assays reveal significant reduction in bacterial burden and enhanced NIH-3T3 fibroblast migration. In vivo diabetic wound models confirm accelerated wound closure, reduced inflammation, enhanced collagen deposition, and neovascularization in infected sites. These findings suggest that the BSA–HA–Curcumin composite hydrogel holds substantial promise as a multifunctional, translational dressing for managing infected diabetic wounds.
