Title : Frontiers of lens-less instrumentation as a fundamental basis for pure scientific, applied biomedical and biotechnological, food agricultural and industrial applications. From know how history to the future
Abstract:
A brief description of the novelty positions and chronology of the research performed by O.V. Gradov group (since 2010 to 2019 - from pure lensless biomicroscopy to the principles of converting non-optical signals into optical ones and diversification of the lensless measurement methods based on different converters and modular features for 3D and multi-angular/multi-axis measurements of the sample characteristics) are presented in the next list:
a. Radio-frequency identification of the cell cultures or tissue cultures on a lensless microscope with wireless signal transmission to a remote receiver was implemented for the first time. This type of identification, which can also be implemented in the analog form, is sensu latoa direct identification using the frequency response of the signal from the individual spectrozonal sensitive elements (2011).
b. Lensless microscopes (including those with Hele-Shaw cells) were used for the first time to monitor reaction-diffusion phenomena and chemical oscillations on a chip (2011).
c. Three- or five-axis lensless holographic microscope based on the universal Fedorov stage and dynamic arm-manipulators with the arbitrary set scanning trajectories - novel robotic research tools for laboratories on a chip (2011-2012) were used for the first time.
d. Universal lensless readers-counters for slides and cell counting chambers (such as Fuchs-Rosenthal, Buerker, Neubauer, Makler type, etc.) have been developed (2012).
e. Telemetric lensless laboratories on a chip, replacing the old Rossi-Cholodny plates/chambers and Perfiliev capillaries, were designed for the first time for real-time soil microbiology studies without removing from the soil, additional processing and microscopy (2012).
f. Techniques of tensoresistor-based microfluidics/strain-gauge lensless detectors as specialized labs-on-a-chip for soil mechanics and foundation monitoring were tested for the first time (2013).
g. Lensless on-chip microscopy systems for food product analysis with automatic calibration using spectro-photometric or colorimetric temperature and chemometric analyte classification were developed (2014-2016).
h. Systems integrating FRAP methods on a chip with lensless microscopy have been created (2015).
i. For the first time labs-on-a-chip with multilevel signal conversion during analog lensless data processing on position-sensitive film and mosaic converters were proposed (2015-2016). For example, magnetographic lensless imaging system were proposed. Lens-less lab-on-a-chip based on the chemooptical + chemotron circuits above the photosensitive elements (that convert / change locally the optical density with changes in the chemical properties of the medium) were proposed.
j. For the first time template lensless biochemical analyzers with carriers based on quartz or mica, and other prebiological mineral substrates were proposed for lensless observation of the templating processes (2016).
k. For the first time integration of lensless microscopy with microelectrode arrays for electrophysiology, which simultaneously perform the function of diffraction gratings or ptychographic aperture arrays, was proposed (2017).
l. Integration of lensless microscopy with MALDI on discrete 2D meander elements or ptychographic aperture arrays on a chip was implemented for the first time (2017).
m. The behavior of ferrofluids was analyzed for the first time on a lensless microscope on a chip (2017-2018).
n. Combustion/oxidation process in air was first observed on a microscopic slide using a lensless microscope (2017-2018).
o. In incoherent lensless 3D hemocytometry on a chip a photometric model based on Lomme-Seeliger law (a physical model of the diffuse reflection) was implemented (2018).
p. Studies on multi-axis goniometric 3D-visualization of the vector field diagrams of optical characteristics of dispersed and biological structures on a chip using different laser scanning regimes and trajectories (which is relevant for the flow analysis of bioparticles) were completed (2018).
And what's the next stage of the technology development?
Unfortunately, the work cycle was terminated due to the merging of institutions and the staff reduction in Russia.
