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4th Edition of International Conference on Tissue Engineering and Regenerative Medicine

September 19-21 | Rome, Italy

September 19 -21, 2024 | Rome, Italy

2D and 3D Tissue Growth

2D and 3D Tissue Growth

Cell culture is a commonly utilized in vitro approach for furthering our understanding of cell biology, tissue morphology, and disease causes, as well as medication action, protein production, and tissue engineering development. The majority of cancer biology research is conducted in vitro utilizing two-dimensional (2D) cell cultures. 2D cultures, on the other hand, have a number of drawbacks, including disruption of interactions between the cellular and extracellular environments, changes in cell shape, polarity, and division mechanism. These drawbacks prompted the development of models that are better suited to simulate in vivo situations. Three-dimensional culture is one such way (3D). Optimising culture conditions could lead to a better knowledge of cancer biology and make biomarkers and targeting medicines more accessible.

Most analysts are familiar with the traditional approach of 2D cell culture, which has been used since the early 1900s. On a flat surface, such as the bottom of a petri dish or flask, it entails securing, nourishing, and developing cell cultures. 2D cell culture systems are well-established and well-proven — they're used in practically all current standard experiments and have a large body of literature to back them up. Because most existing procedures are based on the 2D culture model, 2D cultures and related equipment are very inexpensive and simple to operate. However, due to the fact that cells in the body do not typically grow in a 2D form, substantial work with 2D culture systems in both research and production has revealed that the technique can have difficulties.

3D culture settings are more similar to a cell's natural environment; therefore, they can provide more physiologically meaningful data, which enhances cell culture accuracy and flexibility. A vessel, culture media, and, in many situations, a scaffold, as well as the appropriate cell feeding and incubation conditions, are necessary to support cell development in three dimensions. A vessel and culture conditions capable of supporting three-dimensional cell formation and growth can open up a whole new range of uses.

Committee Members
Speaker at Tissue Engineering and Regenerative Medicine 2024 - A C Matin

A C Matin

Stanford University School of Medicine, United States
Speaker at Tissue Engineering and Regenerative Medicine 2024 - Yong Xiao Wang

Yong Xiao Wang

Albany Medical College, United States
Speaker at Tissue Engineering and Regenerative Medicine 2024 - Berislav V Zlokovic

Berislav V Zlokovic

Zilkha Neurogenetic Institute, United States
TERMC 2024 Speakers
Speaker at Tissue Engineering and Regenerative Medicine 2024 - Patrizia Ferretti

Patrizia Ferretti

UCL Great Ormond Street Institute of Child Health, United Kingdom
Speaker at Tissue Engineering and Regenerative Medicine 2024 - Hossein Hosseinkhani

Hossein Hosseinkhani

Innovation Center for Advanced Technology, Matrix HT, United States
Speaker at Tissue Engineering and Regenerative Medicine 2024 - Vasiliki E Kalodimou

Vasiliki E Kalodimou

Director Flow Cytometry-Research, Greece
Speaker at Tissue Engineering and Regenerative Medicine 2024 - Sergey Suchkov

Sergey Suchkov

The Russian University of Medicine, Russian Federation

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