Bioprinting technology is a powerful tool for generating functional tissues and organs from cells, biological inks based on biological materials, and growth factors in a computer control platform. The latest advances in bioprinting of nerves, skin, heart, bone, cartilage, skeletal muscle and other soft tissues highlight the challenges in these applications. Various composite bio-inks with bioactive ceramic-based scaffolds with patient-specific designs and controllable microstructures have been successfully used in clinical and preclinical applications to regenerate bones. In nerve tissue engineering, the bioprinting of alginate and gelatin-based gel bio-inks by extrusion can present a controllable 3D microstructure, and show satisfactory cell compatibility and axon regeneration. The application of biosignatures in other types of tissues was also studied, and the results showed great potential in the regeneration of tissues such as cornea, liver and bladder.