Cell culture is a term used to describe laboratory techniques that allow eukaryotic or prokaryotic cells to develop in physiological settings. It was first used to research tissue growth and maturation, virus biology and vaccine creation, the function of genes in illness and health, and the utilization of large-scale hybrid cell lines to manufacture biopharmaceuticals in the early twentieth century. Cultured cells have as many experimental uses as there are cell types that can be produced in vitro. Cell culture is most typically used in clinical settings to create model systems for studying basic cell biology, replicating disease mechanisms, or testing the toxicity of new therapeutic molecules. The ability to modify genes and molecular pathways is one of the benefits of employing cell culture for these applications. Furthermore, the homogeneity of clonal cell populations or specific cell types, as well as well-defined culture systems, eliminates interfering genetic or environmental variables, allowing for high reproducibility and consistency in data generation that is impossible to guarantee when studying whole organ systems. In the exploration of basic scientific and translational research concerns, cell culture is a very versatile technique. The uniformity of cell lines and the associated reproducibility of data obtained are advantages of using them in scientific study.