In a lab dish, a stem cell line is a group of identical stem cells that may be grown and fostered. A line starts with a single cell or a group of cells, and each subsequent cell in the line is a duplicate of the original cells. These lines allow researchers to generate vast amounts of cells. They can even store some for later use or to share with colleagues by freezing them in liquid nitrogen. Until now, the methods utilized to find superior culture conditions for the preservation of legitimate stem cells have primarily been empirical. While the empirical approach has yielded some successes, such as the development of ESC and EpiSC culture conditions, the majority of such attempts have failed, and we still don't know how to maintain the long-term self-renewal of the majority of stem cells from various tissue origins in various species. Stem cells have a lot of promise for tissue regeneration and repair. Before the full potential of stem cells may be realized, various hurdles must be overcome. One of the major roadblocks is the lack of well-established strategies for long-term stem cell growth, particularly tissue-specific stem cells.