Generative Tissues

Generative Tissues, or gTissues, represent a specialized category of living tissue in biology. They are surgically created, cultivated within a patient, and possess a distinctive cellular and biochemical composition that sets them apart from other bodily tissues. The inception of gTissues emerged from advancements in Tissue Engineering and Regenerative Medicine, initially observed during the reconstruction of canine dura mater, resulting in the formation of a dense connective tissue between the brain and skull.[1] Subsequently, this technology has been successfully employed in humans for diverse clinical applications, particularly in soft tissue healing and repair. The creation of gTissue involves the surgical implantation of specific non-inflammatory Extracellular Matrix (ECM) biomaterials, which are embraced by the host. This process includes the integration of host cells and blood vessels, ultimately transforming the implanted material into a living tissue. Variants of gTissue developed so far predominantly mirror soft connective tissues like dermis, tendon, ligament, and fascia. The generation of gTissue commences with the implantation of cell-free ECM biomaterial, which progressively integrates with the host. Immediately post-implantation, the porous material absorbs blood, initiating a seeding process with circulating stem cells and growth factors crucial for gTissue development. As the generation proceeds, signals from growth factors, cytokines, and the fibrin provisional matrix prompt host cells to repopulate the matrix. Simultaneously, a vascular network is established within the ECM biomaterial to support the metabolic activity of these cells.