Title : MicroRNAs in the composition of platelet- Rich fibrin
Regenerative effectiveness of platelet-rich fibrin has been discussed in previous clinical research, but information about its composition related to cellular and genetic composition is lacking. A three-dimensional fibrin matrix in platelet-rich fibrin attracts and traps significant quantities of T cells, B cells, macrophages, monocytes, dendritic cells, and growth factors. I
Short and compact non-coding RNA molecules are known as microRNAs and are widely researched in many immunology and oncology studies. It has been reported that microRNAs play an essential role in wound healing and inflammatory process and impact diabetic or oncologic patients' wounds.
Twenty samples of various autologous blood products (including platelet-rich fibrin, platelet-rich plasma, platelet-poor plasma made from PRF, and platelet-poor plasma produced from PRP were used in our investigation. Real-time qPCR with an adapted protocol for sample preparation was used to evaluate the Ct value of different microRNAs in every sample of autologous blood products. In this study, we reported the presence of various microRNAs (miR-16, miR-21, miR-146a and miR-155) in the platelet-rich fibrin and other autologous blood products (PRP and PPP) of canine patients and compared their expression to each other. The additional aim of the study included confirmed stability properties in icy conditions (-30) for a prolonged time of one week and one month, which might be a helpful feature for PRF clinical adaptability.
Audeince Take Away:
- The research can be expanded more to look for microRNAs co-dependency in the inflammatory and regenerative process.
- PRF might be used as an artificial carrier of microRNAs for diabetic wounds, which is a practical benefit.
- Protocol for PRF sample preparation for RNA isolation and RT-qPCR analysis might be helpful for other researchers.