Abstract:
Chloroplast biogenesis was studied in the shoot apical meristem (SAM) of tomato by sequencing mRNA from samples isolated through laser microdissection. Intrinsic Chl fluorescence was used as a guide to isolate cells in different developmental stages of chloroplast, through Laser Microdissection.
RNA sequencing was performed with CEL seq, a method that was developed for single cell RNA-seq and can work better on degraded RNA. Data was analyzed by various bioinformatics tools such as Gene Ontology and promoter analysis and protein networks (STRING DB). HT-qPCR experiment (on 190 genes) was performed as a separate experiment on laser microdissected samples. Several known genes exhibited expression pattern as expected. Beside this many important genes were determined based on analysis of DEGs through bioinformatics tools that could be candidates for chloroplast development.
Biography:
Dr Vijay K Dalal is working as Assistant Professor (Botany; Guest) at Dayalbagh Educational Institute (DEI), Agra, India since 2019. At DEI he is working for development of horticulture practices in hydroponics system as well in soil inside polyhouse. He completed his PhD (Biotechnology), working at Jawaharlal Nehru University (JNU), New Delhi, India while being registered at Maharshi Dayanand University, Rohtak, Haryana, India in 2012. At JNU, he studied the effect of water stress in the drought-sensitive rice cultivar. During his three years postodc at Hebrew Univ, Rehovot Campus, Israel, he studied chloroplast biogenesis in tomato shoot apical meristem through RNA-seq-NGS of tissues obtained through Laser Microdissection. For one year, as Research Associate at Hyderabad University, he studied the effect of osmolyte proline in protecting various enzymes. His work has been published in prestigious peer-reviewed International journals such as “Plant Cell and Environment” and "Scientific Reports" and books such as in Springer, Netherlands and Wiley. He is a reviewer in many prestigious International Peer Reviewed Journals e.g. Food and Function, 3Biotech, Journal of Plant Biochemistry and Biophysics, and Photosynthetica.
