High-Throughput Protein Structural Studies

In structural genomics, high-throughput protein expression and purification play a critical role. In fact, the largest impediment to the conversion of protein structure determination to a high-throughput format will most likely be crystallographic-quality protein manufacturing on the scale required to generate tens to hundreds of different proteins every day. Protein expression and purification are restricted in structural biology due to high-throughput efforts. The bulk of gene constructs must, first and foremost, be expressed in a synchronised manner. Second, the purification process used to purify the majority of produced proteins should be as comparable as possible in order to obtain the high-quality material required for structural research. High-throughput proteomics is a rapidly expanding science that allows for the global profiling of proteins in biological systems. These high-throughput technical breakthroughs are enabling investigations at the size of entire systems (e.g., whole cells, cancers, or environmental communities), which is fuelling a revolution in biology. However, only recognising the proteins in a cell is insufficient for comprehending the total system's underlying intricacy and functioning mechanics.

• Mass Spectrometry
• Nuclear Magnetic Resonance
• X-Ray Crystallography