Nutritional metabolic profiling involves analyzing metabolic responses to dietary intake, providing insights into individual nutritional needs and health status. By measuring metabolites in biofluids such as blood, urine, and saliva, researchers can identify specific metabolic patterns associated with dietary habits and health conditions. This profiling helps to understand how different foods and nutrients influence metabolic pathways, which can vary significantly among individuals due to genetic and lifestyle factors. Advances in analytical techniques, such as high-throughput metabolomics, enable comprehensive metabolic assessments, leading to more tailored dietary recommendations and interventions that enhance health and prevent disease. Such personalized approaches hold promise for optimizing nutrition and improving overall well-being.