Nutritional pathway analysis explores how nutrients interact with metabolic pathways, influencing the body's energy production, detoxification processes, and synthesis of essential compounds. Researchers in this field map out the biochemical routes through which vitamins, minerals, and macronutrients affect cellular functions and overall metabolism. For example, the role of B vitamins in the citric acid cycle, which is crucial for energy production, is a key area of study. Nutritional pathway analysis is vital in identifying how nutrient deficiencies or imbalances disrupt normal metabolic functions, leading to issues like fatigue, poor immunity, or neurodegeneration. By understanding these pathways, researchers can recommend dietary changes to support optimal metabolic health and prevent diseases associated with metabolic dysfunctions, offering insights into therapeutic nutrition and functional food development.