The combination of stem-cell-based tissue engineering with gene therapy has the possibility to provide regenerative tissue cells within an atmosphere of optimal regulatory protein expression and would have many benefits in various areas such as the transplantation of skin, cartilage or bone.
Gene therapy to trigger essential production of factors by the cells of the tissue. Gene therapy is classically used to treat diseases involving deficient or mutated proteins by delivering genes that encode intact proteins to target cells. The contest of gene therapy is to target the right gene to the right location in the right cells and express it adequately at the right time while minimizing any adverse reactions.
In-vivo gene transfer
The in-vivo approach for gene delivery consists of the injection of genetic material directly into the host.
Without scaffold viral approach
Vectors for gene delivery can be categorized as viral or non-viral viruses have evolved over long periods of time to host their genetic material into host cells, hence virus-based vectors have been a popular choice in many gene therapy experiments.
Non-viral approach
Non-viral gene delivery systems include physical methods such as electroporation, microinjection, gene gun delivery or the use of uncomplexed naked DNA.
Title : Small activating RNA from concept to phase 2 clinical trials
Nagy Habib, MiNA Therapeutics Ltd, United Kingdom
Title : Mechanical modulation of cell response in 3D bioprinted hydrogels
Ruben F Pereira, Universidade do Porto, Portugal
Title : Electroactive polymer-based smart scaffolds for tissue engineering and regenerative medicine
Federico Carpi, University of Florence, Italy
Title : Graphene “Hastalex®”, butterfly, and stem cells are set to revolutionise the development of human organs.
Alexander Seifalian, NanoRegMed Ltd, London BioScience Innovation Centre, United Kingdom
Title : Design of 3D bioengineered personalized scaffolds to potentiate bone ingrowth and angiogenic network for oral tissues reconstruction
Christiane Salgado , Instituto de Investigação e Inovação em Saúde (i3S)/Instituto Nacional de Engenharia Biomédica (INEB), Brazil
Title : RADA16-I based scaffolds for wound healing and regenerative medicine
Deptula Milena, Laboratory of Tissue Engineering and Regenerative Medicine, Department of Embryology, Medical University of Gdansk, Poland