The combination of stem-cell-based tissue engineering with gene therapy has the possibility to provide regenerative tissue cells within an atmosphere of optimal regulatory protein expression and would have many benefits in various areas such as the transplantation of skin, cartilage or bone.
Gene therapy to trigger essential production of factors by the cells of the tissue. Gene therapy is classically used to treat diseases involving deficient or mutated proteins by delivering genes that encode intact proteins to target cells. The contest of gene therapy is to target the right gene to the right location in the right cells and express it adequately at the right time while minimizing any adverse reactions.
In-vivo gene transfer
The in-vivo approach for gene delivery consists of the injection of genetic material directly into the host.
Without scaffold viral approach
Vectors for gene delivery can be categorized as viral or non-viral viruses have evolved over long periods of time to host their genetic material into host cells, hence virus-based vectors have been a popular choice in many gene therapy experiments.
Non-viral gene delivery systems include physical methods such as electroporation, microinjection, gene gun delivery or the use of uncomplexed naked DNA.