Next-generation sequencing (NGS), massively parallel or deep sequencing is a related term describing DNA sequencing technology that has revolutionized genome research. The development of sequencing-based chromatin analysis technology allows current researchers to experimentally measure the properties of chromatin. NGS is the choice for large-scale genome and transcriptome sequencing. This is because the high-throughput sequencing data generated and output by each instrument is in the gigabyte range, and the cost is compared with the traditional Sanger first-generation sequencing method.
The difference between NGS technology and Sanger method is that they provide large-scale parallel analysis, which can provide ultra-high throughput of multiple samples at a greatly reduced cost. Millions to billions of DNA nucleotides can be sequenced in parallel, which significantly increases yields and minimizes the need for fragment cloning methods used in Sanger Sequencing.
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